ZBTB20

Chr 3AD

zinc finger and BTB domain containing 20

Also known as: DPZF, HOF, ODA-8S, PRIMS, ZNF288

This transcriptional repressor contains an N-terminal BTB/POZ domain and C-terminal zinc finger domain, functioning in neurogenesis, glucose homeostasis, and postnatal growth. Heterozygous loss-of-function mutations cause Primrose syndrome, an autosomal dominant disorder characterized by intellectual disability, distinctive facial features, and metabolic abnormalities. The high constraint scores (pLI 0.97, LOEUF 0.32) indicate this gene is highly intolerant to loss-of-function variants.

GeneReviewsOMIMResearchSummary from RefSeq, OMIM, UniProt, Mechanism
DNmechanismADLOEUF 0.321 OMIM phenotype
Clinical SummaryZBTB20
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Gene-Disease Validity (ClinGen)
Primrose syndrome · ADDefinitive

Definitive — sufficient evidence for diagnostic panels

Population Constraint (gnomAD)
Highly constrained gene — heterozygous loss-of-function variants are very rare in the population (pLI 0.97). One damaged copy is likely sufficient to cause disease.
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Clinical Trials
1 active or recruiting trial — potential therapeutic options may be available
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GeneReview available — ZBTB20
Authoritative clinical overview · Recommended first read
Open GeneReview ↗

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Dual constrained — LoF & missense intolerant
LoF Constraint
0.32LOEUF
pLI 0.974
Z-score 4.02
OE 0.12 (0.060.32)
Highly constrained

Highly LoF-intolerant (top ~10% of genes)

Missense Constraint
4.27Z-score
OE missense 0.45 (0.400.51)
217 obs / 480.5 exp
Constrained

Highly missense-constrained (top ~0.1%)

Observed / Expected Ratios
LoF OE0.12 (0.060.32)
00.351.4
Missense OE0.45 (0.400.51)
00.61.4
Synonymous OE0.90
01.21.6
LoF obs/exp: 3 / 24.4Missense obs/exp: 217 / 480.5Syn Z: 1.12
Curated Mechanism (G2P)Gene2Phenotype (DDG2P) ↗
definitiveZBTB20-related Primrose syndromeDNAD
DN
0.4090th %ile
GOF
0.3094th %ile
LOF
0.75top 10%

This gene has evidence for multiple mechanisms of pathogenicity (loss-of-function and dominant-negative). Both the Badonyi & Marsh prediction and the broader genomic evidence point to loss-of-function as the predominant mechanism. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

LOFprediction above median · LOEUF 0.32
DN1 literature citation

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Literature Evidence

DNAstrogliogenesis is more severely disrupted by a ZBTB20 protein containing dominant mutations linked to Primrose syndrome, suggesting that ZBTB20 acts in concert with other ZBTB proteins that were also affected by the dominant-negative protein to instruct astrogliogenesis.PMID:34351428

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312. Mechanism ranking also informed by gnomAD constraint, ClinVar, and ClinGen data.

ClinVar Variant Classifications

0 submitted variants in ClinVar

Protein Context — Lollipop Plot

ZBTB20 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

3D Protein StructureAlphaFold
Clinical Literature
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