WDR4

Chr 21AR

WDR4 tRNA N7-guanosine methyltransferase non-catalytic subunit

Also known as: GAMOS6, MIGSB, TRM82, TRMT82, Wuho, hWH

WDR4 encodes a non-catalytic component of the METTL1-WDR4 methyltransferase complex that acts as a scaffold for tRNA binding and is required for N(7)-methylguanine formation in tRNAs, mRNAs, and microRNAs. Mutations cause autosomal recessive Galloway-Mowat syndrome 6 and a distinct phenotype of microcephaly, growth deficiency, seizures, and brain malformations. The gene is highly constrained against loss-of-function variants (LOEUF 1.116), consistent with severe developmental phenotypes when disrupted.

Summary from RefSeq, OMIM, UniProt
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Primary Disease Associations & Inheritance

Galloway-Mowat syndrome 6MIM #618347
AR
Microcephaly, growth deficiency, seizures, and brain malformationsMIM #618346
AR
0
Active trials
31
Pubs (1 yr)
118
P/LP submissions
5%
P/LP missense
1.12
LOEUF
LOF
Mechanism· G2P
Clinical SummaryWDR4
Population Constraint (gnomAD)
Low constraint (pLI 0.00) — loss-of-function variants are relatively tolerated in the population.
📋
ClinVar Variants
105 unique Pathogenic / Likely Pathogenic· 148 VUS of 455 total submissions

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint
1.12LOEUF
pLI 0.000
Z-score 1.16
OE 0.72 (0.481.12)
Tolerant

Highly tolerant — LoF variants common in population

Missense Constraint
-1.02Z-score
OE missense 1.18 (1.071.30)
304 obs / 257.9 exp
Tolerant

Tolerant to missense variation

Observed / Expected Ratios
LoF OE0.72 (0.481.12)
00.351.4
Missense OE1.18 (1.071.30)
00.61.4
Synonymous OE1.18
01.21.6
LoF obs/exp: 15 / 20.7Missense obs/exp: 304 / 257.9Syn Z: -1.47
Curated Mechanism (G2P)Gene2Phenotype (DDG2P) ↗
limitedWDR4-related Galloway-Mowat syndromeLOFAR

Predictions shown for reference only — model trained on dominant genes, not applicable to AR conditions.

DN
0.6357th %ile
GOF
0.4875th %ile
LOF
0.3842th %ile

The Badonyi & Marsh prediction model was trained exclusively on dominant disease genes. Predictions are not reliable for genes with autosomal recessive inheritance and are shown at reduced opacity for reference only.

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

455 submitted variants in ClinVar

Classification Summary

Pathogenic93
Likely Pathogenic12
VUS148
Likely Benign136
Benign38
Conflicting7
93
Pathogenic
12
Likely Pathogenic
148
VUS
136
Likely Benign
38
Benign
7
Conflicting

Curated Variants Distribution

Classified variants from ClinVar · 5 ACMG categories

ClassificationLoFMissense + InframeNon-codingSynonymousTotal
Pathogenic
5
1
87
0
93
Likely Pathogenic
4
4
4
0
12
VUS
2
127
19
0
148
Likely Benign
2
13
49
72
136
Benign
0
3
29
6
38
Conflicting
7
Total1314818878434

LoF = frameshift, stop gained/lost, canonical splice · Counts from ClinVar esearch · Updated hourly

View in ClinVar →

Protein Context — Lollipop Plot

WDR4 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

3D Protein StructureAlphaFold

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

No active trials found for this gene.

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Clinical Literature
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