USP9Y

Chr YYL

ubiquitin specific peptidase 9 Y-linked

Also known as: DFFRY, FAF-Y, SPGFY2

This gene encodes a deubiquitinase that cleaves ubiquitin from target proteins and may stabilize proteins important for male germ cell development. Mutations cause Y-linked spermatogenic failure, which results in male infertility due to defective sperm production. The condition follows Y-linked inheritance, meaning it is passed from father to son through the Y chromosome.

Summary from RefSeq, OMIM, UniProt
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Primary Disease Associations & Inheritance

Spermatogenic failure, Y-linked, 2MIM #415000
YL
0
Active trials
9
Pubs (1 yr)
65
P/LP submissions
P/LP missense
0.59
LOEUF
Multiple*
Mechanism· predicted
Clinical SummaryUSP9Y
Population Constraint (gnomAD)
Low constraint (pLI 0.00) — loss-of-function variants are relatively tolerated in the population.
📋
ClinVar Variants
65 unique Pathogenic / Likely Pathogenic· 25 VUS of 256 total submissions
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GeneReview available — USP9Y
Authoritative clinical overview · Recommended first read
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Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Moderate LoF intolerance
LoF Constraint
0.59LOEUF
pLI 0.000
Z-score 3.52
OE 0.38 (0.250.59)
Moderately constrained

More LoF-intolerant than ~75% of genes

Missense Constraint
-0.39Z-score
OE missense 1.06 (0.971.15)
409 obs / 387.3 exp
Tolerant

Tolerant to missense variation

Observed / Expected Ratios
LoF OE0.38 (0.250.59)
00.351.4
Missense OE1.06 (0.971.15)
00.61.4
Synonymous OE1.15
01.21.6
LoF obs/exp: 14 / 37.2Missense obs/exp: 409 / 387.3Syn Z: -1.35
DN
0.75top 25%
GOF
0.6638th %ile
LOF
0.2969th %ile

This gene has evidence for multiple mechanisms of pathogenicity (dominant-negative and gain-of-function). Both the Badonyi & Marsh prediction and the broader genomic evidence point to dominant-negative as the predominant mechanism. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

DNprediction above median
GOFprediction above median

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

256 submitted variants in ClinVar

Classification Summary

Pathogenic62
Likely Pathogenic3
VUS25
Likely Benign11
Benign2
62
Pathogenic
3
Likely Pathogenic
25
VUS
11
Likely Benign
2
Benign

Curated Variants Distribution

Classified variants from ClinVar · 5 ACMG categories· variant type breakdown unavailable

ClassificationLoFMissense + InframeNon-codingSynonymousTotal
Pathogenic
62
Likely Pathogenic
3
VUS
25
Likely Benign
11
Benign
2
Total103

Counts from ClinVar esearch · Updated hourly

View in ClinVar →

Protein Context — Lollipop Plot

USP9Y · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

No active trials found for this gene.

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Clinical Literature
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