USP40

Chr 2

ubiquitin specific peptidase 40

USP40 encodes a deubiquitinating enzyme that maintains endothelial cell barrier integrity by stabilizing tight junction proteins like Claudin-1, regulates apoptosis through CFLAR stabilization, and suppresses inflammation by inhibiting NF-kappa-B activation. Mutations cause a neurodevelopmental disorder with intellectual disability, developmental delay, and seizures, inherited in an autosomal recessive pattern. The gene shows minimal constraint against loss-of-function variants, consistent with recessive inheritance where heterozygous carriers are typically unaffected.

Summary from RefSeq, UniProt
Research Assistant →
0
Active trials
4
Pubs (1 yr)
36
P/LP submissions
0%
P/LP missense
0.93
LOEUF
DN
Mechanism· predicted
Clinical SummaryUSP40
Population Constraint (gnomAD)
Low constraint (pLI 0.00) — loss-of-function variants are relatively tolerated in the population.
📋
ClinVar Variants
36 unique Pathogenic / Likely Pathogenic· 193 VUS of 285 total submissions

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint
0.93LOEUF
pLI 0.000
Z-score 2.06
OE 0.73 (0.580.93)
Tolerant

Typical tolerance to LoF variation

Missense Constraint
0.24Z-score
OE missense 0.97 (0.911.04)
611 obs / 628.0 exp
Tolerant

Mild missense constraint

Observed / Expected Ratios
LoF OE0.73 (0.580.93)
00.351.4
Missense OE0.97 (0.911.04)
00.61.4
Synonymous OE0.97
01.21.6
LoF obs/exp: 49 / 67.2Missense obs/exp: 611 / 628.0Syn Z: 0.33
DN
0.6646th %ile
GOF
0.5563th %ile
LOF
0.3068th %ile

The highest-scoring mechanism for this gene is dominant-negative.

DNprediction above median

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

285 submitted variants in ClinVar

Classification Summary

Pathogenic35
Likely Pathogenic1
VUS193
Likely Benign12
Benign2
35
Pathogenic
1
Likely Pathogenic
193
VUS
12
Likely Benign
2
Benign

Curated Variants Distribution

Classified variants from ClinVar · 5 ACMG categories

ClassificationLoFMissense + InframeNon-codingSynonymousTotal
Pathogenic
0
0
35
0
35
Likely Pathogenic
0
0
1
0
1
VUS
1
181
11
0
193
Likely Benign
0
11
0
1
12
Benign
0
0
0
2
2
Total1192473243

LoF = frameshift, stop gained/lost, canonical splice · Counts from ClinVar esearch · Updated hourly

View in ClinVar →

Protein Context — Lollipop Plot

USP40 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

No active trials found for this gene.

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Clinical Literature
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