RSPO2

Chr 8AR

R-spondin 2

Also known as: CRISTIN2, HHRRD, TETAMS2

The protein is a secreted ligand that activates canonical Wnt signaling by binding to LGR4-6 receptors and plays a crucial role in embryonic limb specification. Autosomal recessive mutations cause tetraamelia syndrome 2 (absence of all four limbs) and humerofemoral hypoplasia with radiotibial ray deficiency, representing severe limb malformation disorders. The gene shows tolerance to loss-of-function variants in the general population (low pLI), consistent with its recessive inheritance pattern.

Summary from RefSeq, OMIM, UniProt
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Primary Disease Associations & Inheritance

?Humerofemoral hypoplasia with radiotibial ray deficiencyMIM #618022
AR
Tetraamelia syndrome 2MIM #618021
AR
0
Active trials
25
Pubs (1 yr)
44
P/LP submissions
2%
P/LP missense
0.76
LOEUF
LOF
Mechanism· G2P
Clinical SummaryRSPO2
Population Constraint (gnomAD)
Low constraint (pLI 0.02) — loss-of-function variants are relatively tolerated in the population.
📋
ClinVar Variants
43 unique Pathogenic / Likely Pathogenic· 56 VUS of 151 total submissions

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint
0.76LOEUF
pLI 0.025
Z-score 2.19
OE 0.36 (0.190.76)
Tolerant

Typical tolerance to LoF variation

Missense Constraint
0.50Z-score
OE missense 0.88 (0.761.02)
121 obs / 137.4 exp
Tolerant

Mild missense constraint

Observed / Expected Ratios
LoF OE0.36 (0.190.76)
00.351.4
Missense OE0.88 (0.761.02)
00.61.4
Synonymous OE1.24
01.21.6
LoF obs/exp: 5 / 13.8Missense obs/exp: 121 / 137.4Syn Z: -1.26
Curated Mechanism (G2P)Gene2Phenotype (DDG2P) ↗
strongRSPO2-related tetraamelia with lung agenesisLOFAR

Predictions shown for reference only — model trained on dominant genes, not applicable to AR conditions.

DN
0.6744th %ile
GOF
0.5465th %ile
LOF
0.3550th %ile

The Badonyi & Marsh prediction model was trained exclusively on dominant disease genes. Predictions are not reliable for genes with autosomal recessive inheritance and are shown at reduced opacity for reference only.

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

151 submitted variants in ClinVar

Classification Summary

Pathogenic41
Likely Pathogenic2
VUS56
Likely Benign40
Benign9
41
Pathogenic
2
Likely Pathogenic
56
VUS
40
Likely Benign
9
Benign

Curated Variants Distribution

Classified variants from ClinVar · 5 ACMG categories

ClassificationLoFMissense + InframeNon-codingSynonymousTotal
Pathogenic
0
1
40
0
41
Likely Pathogenic
2
0
0
0
2
VUS
1
48
7
0
56
Likely Benign
0
2
9
29
40
Benign
0
1
6
2
9
Total3526231148

LoF = frameshift, stop gained/lost, canonical splice · Counts from ClinVar esearch · Updated hourly

View in ClinVar →

Protein Context — Lollipop Plot

RSPO2 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

No active trials found for this gene.

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Clinical Literature
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