RB1

Chr 13ADSomatic

RB transcriptional corepressor 1

Also known as: OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110

The protein functions as a tumor suppressor that negatively regulates the cell cycle by binding transcription factor E2F1 in its active hypophosphorylated state and stabilizes constitutive heterochromatin to maintain chromatin structure. Mutations cause retinoblastoma and trilateral retinoblastoma through loss of function, inherited in an autosomal dominant pattern with high penetrance. The protein is highly intolerant to loss-of-function variants, consistent with its critical role as a tumor suppressor in childhood cancer.

GeneReviewsOMIMResearchSummary from RefSeq, OMIM, UniProt, Mechanism
LOFmechanismAD/SomaticLOEUF 0.137 OMIM phenotypes
Clinical SummaryRB1
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Gene-Disease Validity (ClinGen)
retinoblastoma · ADDefinitive

Definitive — sufficient evidence for diagnostic panels

Population Constraint (gnomAD)
Highly constrained gene — heterozygous loss-of-function variants are very rare in the population (pLI 1.00). One damaged copy is likely sufficient to cause disease.
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ClinVar Variants
52 unique Pathogenic / Likely Pathogenic· 195 VUS of 400 total submissions
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Clinical Trials
12 active or recruiting trials — potential therapeutic options may be available
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GeneReview available — RB1
Authoritative clinical overview · Recommended first read
Open GeneReview ↗

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

LoF intolerant — likely haploinsufficient
LoF Constraint
0.13LOEUF
pLI 1.000
Z-score 6.93
OE 0.05 (0.020.13)
Highly constrained

Among the most LoF-intolerant genes (~top 3%)

Missense Constraint
2.67Z-score
OE missense 0.65 (0.600.72)
311 obs / 474.9 exp
Mild constraint

Moderately missense-constrained (top ~2.5%)

Observed / Expected Ratios
LoF OE0.05 (0.020.13)
00.351.4
Missense OE0.65 (0.600.72)
00.61.4
Synonymous OE0.95
01.21.6
LoF obs/exp: 3 / 61.8Missense obs/exp: 311 / 474.9Syn Z: 0.49
Curated Mechanism (G2P)Gene2Phenotype (DDG2P) ↗
definitiveRB1-related retinoblastomaLOFAD
DN
0.2997th %ile
GOF
0.2597th %ile
LOF
0.78top 5%

The highest-scoring mechanism for this gene is loss-of-function (haploinsufficiency).

LOFprediction above median · 1 literature citation · 88% of P/LP variants are LoF · LOEUF 0.13

Literature Evidence

LOFHaploinsufficiency of RB1 accelerates cancer pathogenesis in concert with inactivation of tumor protein p53.PMID:27308386

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312. Mechanism ranking also informed by gnomAD constraint, ClinVar, and ClinGen data.

ClinVar Variant Classifications

400 submitted variants in ClinVar

Classification Summary

Pathogenic46
Likely Pathogenic6
VUS195
Likely Benign112
Benign2
Conflicting1
46
Pathogenic
6
Likely Pathogenic
195
VUS
112
Likely Benign
2
Benign
1
Conflicting

Curated Variants Distribution

Classified variants from ClinVar · 5 ACMG categories

ClassificationLoFMissense + InframeNon-codingSynonymousTotal
Pathogenic
43
1
2
0
46
Likely Pathogenic
3
1
2
0
6
VUS
0
127
67
1
195
Likely Benign
0
3
61
48
112
Benign
0
0
2
0
2
Conflicting
1
Total4613213449362

LoF = frameshift, stop gained/lost, canonical splice · Counts from ClinVar esearch · Updated hourly

View in ClinVar →

Protein Context — Lollipop Plot

RB1 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

3D Protein StructureAlphaFold

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

Endometrial Cancer

A Study of Targeted Agents for Patients With Recurrent or Persistent Endometrial Cancer

RECRUITING
NCT04486352Phase PHASE1, PHASE2Alliance Foundation Trials, LLC.Started 2021-10-20
Atezolizumab - 28 Day CycleBevacizumabIpatasertib
SarcomaHER-2 Protein OverexpressionOsteosarcoma

HER2 Chimeric Antigen Receptor (CAR) T Cells in Combination With Checkpoint Blockade in Patients With Advanced Sarcoma

RECRUITING
NCT04995003Phase PHASE1Baylor College of MedicineStarted 2021-12-07
T cells or CAR T cellsPembrolizumab Injectable ProductNivolumab Injectable Product
Lung Cancer

Feasibility of Targeted Bronchial Washing for Molecular Testing by Next Generation Sequencing in Early-stage Lung Cancer

ACTIVE NOT RECRUITING
NCT06301295Phase NAPusan National University HospitalStarted 2024-05-29
Ultarthin bronchoscopy with intratumoral washing
Solid TumorsCNS Tumors

Study Of Entrectinib (Rxdx-101) in Children and Adolescents With Locally Advanced Or Metastatic Solid Or Primary CNS Tumors And/Or Who Have No Satisfactory Treatment Options

ACTIVE NOT RECRUITING
NCT02650401Phase PHASE1, PHASE2Hoffmann-La RocheStarted 2016-05-03
Entrectinib
Malignant Solid NeoplasmRecurrent Adrenal Gland PheochromocytomaRecurrent Ectomesenchymoma

Tipifarnib for the Treatment of Advanced Solid Tumors, Lymphoma, or Histiocytic Disorders With HRAS Gene Alterations, a Pediatric MATCH Treatment Trial

ACTIVE NOT RECRUITING
NCT04284774Phase PHASE2National Cancer Institute (NCI)Started 2020-10-13
Tipifarnib
Hematopoietic and Lymphatic System NeoplasmRecurrent EpendymomaRecurrent Ewing Sarcoma

Selpercatinib for the Treatment of Advanced Solid Tumors, Lymphomas, or Histiocytic Disorders With Activating RET Gene Alterations, a Pediatric MATCH Treatment Trial

ACTIVE NOT RECRUITING
NCT04320888Phase PHASE2National Cancer Institute (NCI)Started 2021-05-03
Computed TomographyMagnetic Resonance ImagingPositron Emission Tomography
Refractory NeuroblastomaRelapsed NeuroblastomaHigh-risk Neuroblastoma

GPC2 CAR T Cells for Relapsed or Refractory Neuroblastoma and Metastatic Retinoblastoma

RECRUITING
NCT05650749Phase PHASE1Stephan Grupp MD PhDStarted 2023-05-23
GPC2 CAR T cells
NeuroblastomaEwing Sarcoma

Fertility Preservation in Children With Solid Tumors: Detection of Residual Disease by a Sensitive Method

RECRUITING
NCT07141862University Hospital, Clermont-FerrandStarted 2025-02-01
RT-qPCRddPCR
Advanced OsteosarcomaRecurrent OsteosarcomaRefractory Osteosarcoma

Genetically Engineered Cells (FH-FOLR1 ST CAR T Cells) for the Treatment of Advanced Refractory or Recurrent/Progressive Osteosarcoma, FIERCe Trial

RECRUITING
NCT07227571Phase PHASE1Fred Hutchinson Cancer CenterStarted 2026-01-16
FH FOLR1 ST CAR T-cellsLeukapheresisFludarabine
Relapsed NeuroblastomaRefractory NeuroblastomaRelapsed Osteosarcoma

NKG2D.Zeta-NK Cell Conditioning With C7R.GD2.CAR-T Cells for Patients With Relapsed or Refractory Osteosarcoma or Neuroblastoma

NOT YET RECRUITING
NCT07211737Phase PHASE1Baylor College of MedicineStarted 2026-05
i15.NKG2D.zeta NK cells and C7R.GD2.CARTs cellsi15.NKG2D.zeta NK cells and C7R.GD2.CARTs cellsi15.NKG2D.zeta NK cells and C7R.GD2.CARTs cells
Laryngeal Carcinoma

The Relationship Between The Differential Expression Of FosB Protein In Laryngeal Cancer Tissues And Clinical Prognosis

NOT YET RECRUITING
NCT06836765Yang jiStarted 2025-04-01
the expression negative in laryngeal cancer tissuethe expression positive in laryngeal cancer tissue
Prostate Cancer Metastatic

Pembrolizumab, Carboplatin and Cabazitaxel in Aggressive Metastatic Castration Resistant Prostate Cancer (PEAPOD_FOS)

RECRUITING
NCT05563558Phase PHASE2Fundacion OncosurStarted 2023-05-05
PembrolizumabCarboplatinCabazitaxel
Clinical Literature
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