RAD51

Chr 15ADSomatic

RAD51 recombinase

Also known as: BRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2, RAD51A, RECA

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

GeneReviewsOMIMResearchGenerating clinical summary…
LOFmechanismAD/SomaticLOEUF 0.643 OMIM phenotypes
Clinical SummaryRAD51
Population Constraint (gnomAD)
Constrained for loss-of-function variants (OE-LoF 0.33) despite low pLI — interpret in context.
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ClinVar Variants
14 unique Pathogenic / Likely Pathogenic· 247 VUS of 505 total submissions
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Clinical Trials
12 active or recruiting trials — potential therapeutic options may be available
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GeneReview available — RAD51
Authoritative clinical overview · Recommended first read
Open GeneReview ↗

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint?
0.64LOEUF
pLI 0.027
Z-score 2.68
OE 0.33 (0.180.64)
Tolerant

Typical tolerance to LoF variation

Missense Constraint?
2.62Z-score
OE missense 0.45 (0.380.54)
81 obs / 180.0 exp
Mild constraint

Moderately missense-constrained (top ~2.5%)

Observed / Expected Ratios?
LoF OE?0.33 (0.180.64)
00.351.4
Missense OE?0.45 (0.380.54)
00.61.4
Synonymous OE?1.10
01.21.6
LoF obs/exp: 6 / 18.4Missense obs/exp: 81 / 180.0Syn Z: -0.63
Curated Mechanism (G2P)Gene2Phenotype (DDG2P) ↗
limitedRAD51-related mirror movementsLOFAD

This gene — mechanism propensity

DN
0.77top 25%
GOF
0.4480th %ile
LOF
0.2968th %ile

This gene has evidence for multiple mechanisms of pathogenicity (dominant-negative and loss-of-function). Both the Badonyi & Marsh prediction and the broader genomic evidence point to dominant-negative as the predominant mechanism. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

DNprediction above median · 1 literature citation
LOF1 literature citation · 29% of P/LP variants are LoF

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Literature Evidence

DNA novel Fanconi anaemia subtype associated with a dominant-negative mutation in RAD51.1
LOFRAD51 haploinsufficiency causes congenital mirror movements in humans.2

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312. Mechanism ranking also informed by gnomAD constraint, ClinVar, and ClinGen data.

ClinVar Variant Classifications

505 submitted variants in ClinVar

Classification Summary

Pathogenic5
Likely Pathogenic9
VUS247
Likely Benign197
Benign28
Conflicting7
5
Pathogenic
9
Likely Pathogenic
247
VUS
197
Likely Benign
28
Benign
7
Conflicting

Curated Variants Distribution

Classified variants from ClinVar · 5 ACMG categories

ClassificationLoFMissense + InframeNon-codingSynonymousTotal
Pathogenic
2
3
0
0
5
Likely Pathogenic
2
7
0
0
9
VUS
6
236
3
2
247
Likely Benign
2
16
36
143
197
Benign
0
2
26
0
28
Conflicting
7
Total1226465145493

LoF = frameshift, stop gained/lost, canonical splice · Counts from ClinVar esearch · Updated hourly

View in ClinVar →

6 pathogenic / likely-pathogenic (of 14) ClinVar copy-number / structural variants overlap RAD51 — these span large chromosomal regions, not the gene specifically, and are excluded from the counts above. Explore in CNV tools →

Protein Context — Lollipop Plot

RAD51 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

Stage III Uterine Corpus Leiomyosarcoma AJCC v8Stage IV Uterine Corpus Leiomyosarcoma AJCC v8Uterine Corpus Leiomyosarcoma

A Phase II Clinical Trial Evaluating the Combination of Olaparib and Temozolomide for the Treatment of Advanced Uterine Leiomyosarcoma

ACTIVE NOT RECRUITING
NCT03880019Phase PHASE2National Cancer Institute (NCI)Started 2019-08-19
Computed TomographyCore BiopsyMagnetic Resonance Imaging
Metastatic Pancreatic Ductal AdenocarcinomaHomologous Recombination Deficiency (HRD)

A Study of Pembrolizumab and Olaparib for People With Metastatic Pancreatic Ductal Adenocarcinoma and Homologous Recombination Deficiency or Exceptional Treatment Response to Platinum-Based Therapy

ACTIVE NOT RECRUITING
NCT04666740Phase PHASE2Memorial Sloan Kettering Cancer CenterStarted 2020-12-18
PembrolizumabOlaparib
Metastatic Breast CancerInvasive Breast CancerSomatic Mutation Breast Cancer (BRCA1)

Olaparib In Metastatic Breast Cancer

ACTIVE NOT RECRUITING
NCT03344965Phase PHASE2Beth Israel Deaconess Medical CenterStarted 2018-04-01
Olaparib
Breast CancerTriple Negative Breast Neoplasms

NordicTrip, a Translational Study of Preoperative Chemotherapy in TNBC

ACTIVE NOT RECRUITING
NCT04335669Phase PHASE3Lund University HospitalStarted 2019-12-20
epirubicin, cyclophosphamide, paclitaxel, carboplatin, pembrolizumabepirubicin, cyclophosphamide, capecitabine, paclitaxel, carboplatin, pembrolizumab
Prostate Cancer Metastatic Castration-ResistantAbnormal DNA RepairMetastatic Prostate Carcinoma

Abiraterone/Prednisone, Olaparib, or Abiraterone/Prednisone + Olaparib in Patients With Metastatic Castration-Resistant Prostate Cancer With DNA Repair Defects

ACTIVE NOT RECRUITING
NCT03012321Phase PHASE2Northwestern UniversityStarted 2017-01-12
OlaparibAbiraterone AcetatePrednisone
Anatomic Stage III Breast Cancer AJCC v8Anatomic Stage IV Breast Cancer AJCC v8Castration-Resistant Prostate Carcinoma

Measuring the Effects of Talazoparib in Patients With Advanced Cancer and DNA Repair Variations

RECRUITING
NCT04550494Phase PHASE2National Cancer Institute (NCI)Started 2021-04-26
Biopsy ProcedureBiospecimen CollectionComputed Tomography
Metastatic Malignant Solid NeoplasmUnresectable Malignant Solid Neoplasm

Studying the Safety and Determining the Optimal Dose of Novobiocin in Patients With Tumors That Have Alterations in DNA Repair Genes

RECRUITING
NCT05687110Phase PHASE1National Cancer Institute (NCI)Started 2023-07-06
Biopsy ProcedureBiospecimen CollectionDiagnostic Imaging Testing
Pancreatic Cancer

Pancreatic Cancer Genetics

RECRUITING
NCT01102569Columbia UniversityStarted 2008-01
Ovarian CancerFallopian Tube CancerPeritoneal Cancer

Microdevice In Ovarian, Fallopian Tube, And Peritoneal Cancer

RECRUITING
NCT04701645Phase PHASE1Brigham and Women's HospitalStarted 2022-11-01
Microdevice
Advanced Fallopian Tube CarcinomaAdvanced Malignant Solid NeoplasmAdvanced Ovarian Carcinoma

Testing the Addition of an Anti-cancer Drug, Elimusertib (BAY 1895344) ATR Inhibitor, to the Chemotherapy Treatment (Gemcitabine) for Advanced Pancreatic and Ovarian Cancer, and Advanced Solid Tumors

ACTIVE NOT RECRUITING
NCT04616534Phase PHASE1National Cancer Institute (NCI)Started 2021-06-01
Biopsy ProcedureBiospecimen CollectionDiagnostic Imaging Testing
Advanced Malignant Solid NeoplasmMetastatic Malignant Solid Neoplasm

Testing How the Body Responds to the Drug CBX-12 in Patients With Advanced Solid Cancers

RECRUITING
NCT05691517Phase PHASE1National Cancer Institute (NCI)Started 2024-06-12
Biopsy ProcedureBiospecimen CollectionComputed Tomography
Metastatic Pancreatic Cancer

Olaparib and Durvalumab (MEDI4736) in Patients with Metastatic Pancreatic Cancer and DNA Damage Repair Genes Alterations

ACTIVE NOT RECRUITING
NCT05659914Phase PHASE2Spanish Cooperative Group for the Treatment of Digestive Tumours (TTD)Started 2022-11-28
olaparib+durvalumab