NDUFAF2

Chr 5AR

NADH:ubiquinone oxidoreductase complex assembly factor 2

Also known as: B17.2L, MC1DN10, MMTN, NDUFA12L, mimitin

The protein functions as an assembly factor for mitochondrial complex I, which catalyzes electron transfer from NADH to ubiquinone in the respiratory chain. Mutations cause mitochondrial complex I deficiency with progressive encephalopathy, inherited in an autosomal recessive pattern. The pathogenic mechanism involves loss of protein function leading to impaired complex I assembly and subsequent mitochondrial dysfunction.

OMIMResearchSummary from RefSeq, OMIM, UniProt, Mechanism
LOFmechanismARLOEUF 1.601 OMIM phenotype
Clinical SummaryNDUFAF2
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Gene-Disease Validity (ClinGen)
Leigh syndrome · ARDefinitive

Definitive — sufficient evidence for diagnostic panels

2 total gene-disease associations curated

Population Constraint (gnomAD)
Low constraint (pLI 0.00) — loss-of-function variants are relatively tolerated in the population.

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint
1.60LOEUF
pLI 0.000
Z-score 0.19
OE 0.93 (0.561.60)
Tolerant

Highly tolerant — LoF variants common in population

Missense Constraint
-0.07Z-score
OE missense 1.02 (0.851.23)
82 obs / 80.3 exp
Tolerant

Tolerant to missense variation

Observed / Expected Ratios
LoF OE0.93 (0.561.60)
00.351.4
Missense OE1.02 (0.851.23)
00.61.4
Synonymous OE1.16
01.21.6
LoF obs/exp: 9 / 9.6Missense obs/exp: 82 / 80.3Syn Z: -0.64
Curated Mechanism (G2P)Gene2Phenotype (DDG2P) ↗
strongNDUFAF2-related Leigh syndromeLOFAR

Predictions shown for reference only — model trained on dominant genes, not applicable to AR conditions.

DN
0.77top 25%
GOF
0.5661th %ile
LOF
0.1796th %ile

The Badonyi & Marsh prediction model was trained exclusively on dominant disease genes. Predictions are not reliable for genes with autosomal recessive inheritance and are shown at reduced opacity for reference only.

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

0 submitted variants in ClinVar

Protein Context — Lollipop Plot

NDUFAF2 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

3D Protein StructureAlphaFold

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

No active trials found for this gene.

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Clinical Literature
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Recent Gene-Specific Literature
Gene in title · MEDLINE · newest first
Europe PMC