MARCHF8

Chr 10

membrane associated ring-CH-type finger 8

Also known as: CMIR, MARCH-VIII, MARCH8, MIR, RNF178, c-MIR

MARCHF8 encodes a membrane-bound E3 ubiquitin ligase that regulates immune responses by ubiquitinating and targeting various proteins including viral envelope proteins, MHC class II molecules, and immune receptors for degradation. Mutations cause autosomal recessive primary immunodeficiency with recurrent infections, growth retardation, and developmental delays typically presenting in early childhood. The gene shows no intolerance to loss-of-function variants based on population genetics data.

Summary from RefSeq, UniProt
Research Assistant →
0
Active trials
9
Pubs (1 yr)
13
P/LP submissions
P/LP missense
1.31
LOEUF
Multiple*
Mechanism· predicted
Clinical SummaryMARCHF8
Population Constraint (gnomAD)
Low constraint (pLI 0.00) — loss-of-function variants are relatively tolerated in the population.
📋
ClinVar Variants
13 unique Pathogenic / Likely Pathogenic· 4 VUS of 24 total submissions

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint
1.31LOEUF
pLI 0.000
Z-score 0.71
OE 0.79 (0.501.31)
Tolerant

Highly tolerant — LoF variants common in population

Missense Constraint
1.04Z-score
OE missense 0.77 (0.670.89)
124 obs / 161.1 exp
Tolerant

Mild missense constraint

Observed / Expected Ratios
LoF OE0.79 (0.501.31)
00.351.4
Missense OE0.77 (0.670.89)
00.61.4
Synonymous OE0.88
01.21.6
LoF obs/exp: 11 / 13.8Missense obs/exp: 124 / 161.1Syn Z: 0.75
DN
0.77top 25%
GOF
0.6736th %ile
LOF
0.48top 25%

This gene has evidence for multiple mechanisms of pathogenicity (dominant-negative and gain-of-function). Both the Badonyi & Marsh prediction and the broader genomic evidence point to dominant-negative as the predominant mechanism. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

DNprediction above median
GOFprediction above median

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

24 submitted variants in ClinVar

Classification Summary

Pathogenic11
Likely Pathogenic2
VUS4
Likely Benign2
Benign1
11
Pathogenic
2
Likely Pathogenic
4
VUS
2
Likely Benign
1
Benign

Curated Variants Distribution

Classified variants from ClinVar · 5 ACMG categories· variant type breakdown unavailable

ClassificationLoFMissense + InframeNon-codingSynonymousTotal
Pathogenic
11
Likely Pathogenic
2
VUS
4
Likely Benign
2
Benign
1
Total20

Counts from ClinVar esearch · Updated hourly

View in ClinVar →

Protein Context — Lollipop Plot

MARCHF8 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

3D Protein StructureAlphaFold

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

No active trials found for this gene.

Search ClinicalTrials.gov →
Clinical Literature
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