LAG3

Chr 12

lymphocyte activating 3

Also known as: CD223

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]

12
Active trials
267
Pubs (1 yr)
0
P/LP submissions
P/LP missense
0.50
LOEUF
Multiple*
Mechanism· predicted
Clinical SummaryLAG3
Population Constraint (gnomAD)
Constrained for loss-of-function variants (OE-LoF 0.24) despite low pLI — interpret in context.
💊
Clinical Trials
12 active or recruiting trials — potential therapeutic options may be available

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Moderate LoF intolerance
LoF Constraint
0.50LOEUF
pLI 0.276
Z-score 3.24
OE 0.24 (0.120.50)
Moderately constrained

More LoF-intolerant than ~75% of genes

Missense Constraint
0.51Z-score
OE missense 0.91 (0.821.01)
246 obs / 269.7 exp
Tolerant

Mild missense constraint

Observed / Expected Ratios
LoF OE0.24 (0.120.50)
00.351.4
Missense OE0.91 (0.821.01)
00.61.4
Synonymous OE1.11
01.21.6
LoF obs/exp: 5 / 21.0Missense obs/exp: 246 / 269.7Syn Z: -0.89
DN
0.6357th %ile
GOF
0.6443th %ile
LOF
0.3647th %ile

This gene has evidence for multiple mechanisms of pathogenicity (gain-of-function and dominant-negative). Both the Badonyi & Marsh prediction and the broader genomic evidence point to gain-of-function as the predominant mechanism. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

GOFprediction above median
DNprediction above median

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

0 submitted variants in ClinVar

Protein Context — Lollipop Plot

LAG3 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

Non-small Cell Lung CancerHepatocellular CarcinomaHead and Neck Squamous Cell Carcinoma

Neoadjuvant Cemiplimab for the Treatment of Resectable NSCLC, HCC, and HNSCC in Adult Patients

ACTIVE NOT RECRUITING
NCT03916627Phase PHASE2Regeneron PharmaceuticalsStarted 2019-07-23
cemiplimabPlatinum Doubletfianlimab
Head and Neck Squamous Cell Carcinoma (HNSCC)

Study of Safety and Tolerability of Nivolumab Treatment Alone or in Combination With Relatlimab or Ipilimumab in Head and Neck Cancer

RECRUITING
NCT04080804Phase PHASE2Dan ZandbergStarted 2019-12-20
NivolumabRelatlimabIpilimumab
Merkel Cell Carcinoma

Neoadjuvant Nivolumab and Relatlimab in Merkel Cell Carcinoma

RECRUITING
NCT06151236Phase PHASE2Melanoma Institute AustraliaStarted 2024-03-11
Nivolumab 240 mg / Relatlimab 80 mg in a fixed dose combination
NSCLCLocally Advanced

Thoracic Radiotherapy and Inhibition of PD-1 and LAG-3 for Locally Advanced Non-Small Cell Lung Cancer

RECRUITING
NCT06865339Phase PHASE2Nitin OhriStarted 2025-08-07
CemiplimabFianlimabRadiotherapy
Salivary Gland TumorBenign Salivary Gland Tumor

Immune Biomarker Study for Salivary Gland Carcinoma

RECRUITING
NCT06047236University of Erlangen-Nürnberg Medical SchoolStarted 2024-01-08
Sampling
Cutaneous Squamous Cell Carcinoma

Neoadjuvant Nivolumab and Relatlimab in Cutaneous Squamous Cell Carcinoma

RECRUITING
NCT06288191Phase PHASE2Melanoma Institute AustraliaStarted 2024-06-21
Nivolumab 240 mg / Relatlimab 80 mg in a fixed dose combination
Solid TumorsMetastatic MelanomaNon-small Cell Lung Cancer

Dose Escalation Study of a PD1-LAG3 Bispecific Antibody in Patients With Advanced and/or Metastatic Solid Tumors

ACTIVE NOT RECRUITING
NCT04140500Phase PHASE1, PHASE2Hoffmann-La RocheStarted 2019-11-11
RO7247669
Melanoma Stage IIIMelanoma Stage IVAdvanced Melanoma

Neoadjuvant and Adjuvant Anti-PD1 or Combinations for Locoregionally Advanced Melanoma

RECRUITING
NCT06295159Phase PHASE2H. Lee Moffitt Cancer Center and Research InstituteStarted 2024-05-17
NivolumabNivolumab + RelatlimabIpilimumab
Multiple Myeloma

A Clinical Study of TQB2029 for Injection in Subjects With Multiple Myeloma

RECRUITING
NCT06700395Phase PHASE1Chia Tai Tianqing Pharmaceutical Group Nanjing Shunxin Pharmaceutical Co., Ltd.Started 2024-11-28
TQB2029 injection
Non-small Cell Lung Cancer

A Trial to Learn How the Combination of Fianlimab With Cemiplimab and Chemotherapy Works Compared With Cemiplimab and Chemotherapy for Treating Adult Patients With Advanced Non-small Cell Lung Cancer

ACTIVE NOT RECRUITING
NCT05800015Phase PHASE2, PHASE3Regeneron PharmaceuticalsStarted 2023-08-08
fianlimabcemiplimabPemetrexed
Breast Cancer MetastaticBreast Carcinoma

Immun Checkpoint Washout in Patients With Invasive Ductal Breast Cancer

RECRUITING
NCT07003009Phase NAIstanbul Training and Research HospitalStarted 2024-01-01
Malign Lymph Node WashoutBenign Lymph Node Washout
Advanced LymphomaAdvanced Malignant Solid NeoplasmBladder Carcinoma

Targeted Therapy Directed by Genetic Testing in Treating Patients With Advanced Refractory Solid Tumors, Lymphomas, or Multiple Myeloma (The MATCH Screening Trial)

ACTIVE NOT RECRUITING
NCT02465060Phase PHASE2National Cancer Institute (NCI)Started 2015-08-17
AdavosertibAfatinibAfatinib Dimaleate
Clinical Literature
Open Research Assistant →
Full-Text Mentions
NLP-detected gene mentions in article bodies · via PubTator3
PubTator3
Lag3: From Bench to Bedside.
Aroldi F et al.·Cancer Treat Res
2022
Overcoming the LAG3 phase problem
Petersen J et al.·Nat Immunol
2022
LAG3's Enigmatic Mechanism of Action
Graydon CG et al.·Front Immunol
2021Functional
Understanding LAG3 immune checkpoint function.
Crunkhorn S·Nat Rev Drug Discov
2025
Top 5 full-text resultsSearch PubTator3 ↗