KRAS

Chr 12ADSomatic

KRAS proto-oncogene, GTPase

Also known as: 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras

The KRAS protein is a small GTPase that regulates cell signaling pathways controlling cell growth and differentiation. Germline mutations cause autosomal dominant neurodevelopmental disorders including Noonan syndrome 3 and cardiofaciocutaneous syndrome 2, while somatic mutations lead to various cancers and RAS-associated autoimmune leukoproliferative disorder. The pathogenic mechanism involves dominant-negative effects from activating mutations, typically single amino acid substitutions that disrupt normal GTPase function.

GeneReviewsOMIMResearchSummary from RefSeq, OMIM, UniProt, Mechanism
GOFmechanismAD/SomaticLOEUF 1.2412 OMIM phenotypes
VCEP Guidelines: RASopathyReleased
View SpecificationsClinGen Panel
Clinical SummaryKRAS
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Gene-Disease Validity (ClinGen)
cardiofaciocutaneous syndrome · ADStrong

Strong evidence — appropriate for clinical testing

3 total gene-disease associations curated

Population Constraint (gnomAD)
Low constraint (pLI 0.00) — loss-of-function variants are relatively tolerated in the population.
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Clinical Trials
12 active or recruiting trials — potential therapeutic options may be available
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GeneReview available — KRAS
Authoritative clinical overview · Recommended first read
Open GeneReview ↗

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint
1.24LOEUF
pLI 0.001
Z-score 1.06
OE 0.63 (0.341.24)
Tolerant

Highly tolerant — LoF variants common in population

Missense Constraint
2.32Z-score
OE missense 0.34 (0.260.45)
33 obs / 97.4 exp
Mild constraint

Moderately missense-constrained (top ~2.5%)

Observed / Expected Ratios
LoF OE0.63 (0.341.24)
00.351.4
Missense OE0.34 (0.260.45)
00.61.4
Synonymous OE0.93
01.21.6
LoF obs/exp: 6 / 9.5Missense obs/exp: 33 / 97.4Syn Z: 0.34
Curated Mechanism (G2P)Gene2Phenotype (DDG2P) ↗
definitiveKRAS-related cardiofaciocutaneous syndromeGOFAD
definitiveKRAS-related Noonan syndromeGOFAD
DN
0.77top 25%
GOF
0.75top 25%
LOF
0.3842th %ile

This gene has evidence for multiple mechanisms of pathogenicity (gain-of-function, dominant-negative and loss-of-function). The Badonyi & Marsh model scores dominant-negative highest among its predictions, but genomic evidence (constraint, ClinVar variant spectrum, and literature) most strongly supports gain-of-function. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

GOFprediction above median · 1 literature citation
DNprediction above median
LOF1 literature citation

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Literature Evidence

GOFActivating germline mutations of KRAS and HRAS cause severe developmental abnormalities leading to Noonan, cardio-facial-cutaneous, and Costello syndrome, but activating germline mutations of NRAS have not been reported.PMID:17517660
LOFHere we report, a syndromic familial case of a 12p duplication encompassing the dosage sensitive gene KRAS, whose phenotype overlapped with rasopathies.PMID:27450488

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

0 submitted variants in ClinVar

Protein Context — Lollipop Plot

KRAS · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

3D Protein StructureAlphaFold

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

OncologyMEK MutationRAF Gene Mutation

A Study to Investigate the Safety and Efficacy of NST-628 Oral Tablets in Subjects With Solid Tumors

RECRUITING
NCT06326411Phase PHASE1Nested Therapeutics, IncStarted 2024-04-09
NST-628
Carcinoma, Pancreatic DuctalColorectal NeoplasmsCarcinoma, Non-Small-Cell Lung

A Study to Learn About the Study Medicine PF-07934040 When Given Alone or With Other Anti-cancer Therapies in People With Advanced Solid Tumors That Have a Genetic Mutation.

RECRUITING
NCT06447662Phase PHASE1PfizerStarted 2024-06-27
PF-07934040GemcitabineNab-paclitaxel
Metastatic Colorectal Cancer (mCRC)Colorectal Neoplasms

Efficacy And Safety Of Hydroxychloroquine Combined With Methotrexate, Capecitabine And Bevacizumab Vs. Regorafenib In Participants With Refractory Metastatic Colorectal Cancer With Mutations In RAS Genes

RECRUITING
NCT06949982Phase PHASE2Sergey Orlov, MDStarted 2025-03-17
experimental group x-MAPRegorafenib (BAY 73-4506)
Melanoma

Clinical Study of Fianlimab in Combination With Cemiplimab Versus Pembrolizumab in Adolescent and Adult Patients With Previously Untreated Unresectable Locally Advanced or Metastatic Melanoma

ACTIVE NOT RECRUITING
NCT05352672Phase PHASE3Regeneron PharmaceuticalsStarted 2022-07-14
FianlimabCemiplimabPembrolizumab
Metastatic Malignant Solid Neoplasm

Autologous CD8+ and CD4+ Transgenic T Cells Expressing High Affinity KRASG12V Mutation-Specific T Cell Receptors (FH-A11KRASG12V-TCR) in Treating Patients With Metastatic Solid Tumor Cancers With KRAS G12V Mutations

RECRUITING
NCT06043713Phase PHASE1Fred Hutchinson Cancer CenterStarted 2023-12-15
BendamustineBiopsyBiospecimen Collection
Solid TumorNon-Small-Cell Lung CancerPancreatic Ductal Adenocarcinoma

A Study to Find a Suitable Dose of ASP5834 in Adults With Solid Tumors

RECRUITING
NCT07094204Phase PHASE1Astellas Pharma IncStarted 2025-07-25
ASP5834panitumumab
Pancreas Cancer

ctDNA Assay in Patients With Resectable Pancreatic Cancer

RECRUITING
NCT05052671University of OklahomaStarted 2022-05-25
Fibrostenotic Crohn's Disease

STENOVA - A Study to Evaluate Safety, Tolerability, PK and PD of AGMB-129 in Patients With Fibrostenotic Crohn's Disease

ACTIVE NOT RECRUITING
NCT05843578Phase PHASE2Agomab Spain S.L.U.Started 2023-08-01
AGMB-129Placebo
PDAC

Master Protocol of TCR-modified T Cell Therapy Targeting HLA-restricted KRAS Antigen Administered in Adult Patients With Metastatic or Locally Advanced PDAC

RECRUITING
NCT07145450Phase PHASE1, PHASE2Anocca ABStarted 2025-07-03
ANOC-001 (TCR-T cells targeting KRAS G12V mutation presented by specific HLA alleles)ANOC-002 (TCR-T cells targeting KRAS G12V mutation presented by specific HLA alleles)ANOC-003 (TCR-T cells targeting KRAS G12D mutation presented by specific HLA alleles)
Lung Cancer

Feasibility of Targeted Bronchial Washing for Molecular Testing by Next Generation Sequencing in Early-stage Lung Cancer

ACTIVE NOT RECRUITING
NCT06301295Phase NAPusan National University HospitalStarted 2024-05-29
Ultarthin bronchoscopy with intratumoral washing
Locally Advanced Lung Non-Small Cell CarcinomaMetastatic Lung Non-Small Cell CarcinomaStage III Lung Cancer AJCC v8

Sotorasib in Combination With Trastuzumab Deruxtecan for the Treatment of Locally Advanced and Metastatic Non-small Cell Lung Cancer With a KRAS G12C Mutation

RECRUITING
NCT07012031Phase PHASE1, PHASE2National Cancer Institute (NCI)Started 2026-08-07
Biopsy ProcedureBiospecimen CollectionComputed Tomography
Non-small Cell Lung CancerHistiocytic NeoplasmHistiocytosis

Phase 1/2 Trial of S241656 in Selected RAS/MAPK Mutation- Positive Malignancies

RECRUITING
NCT05786924Phase PHASE1, PHASE2Institut de Recherches Internationales ServierStarted 2023-04-18
S241656FOLFOX6/FOLFOX7FOLFIRI
Clinical Literature
Open Research Assistant →
Full-Text Mentions
NLP-detected gene mentions in article bodies · via PubTator3
PubTator3
Top 5 full-text resultsSearch PubTator3 ↗
Key Publications
Landmark & review papers · by relevance
PubMed
KRAS mutation: from undruggable to druggable in cancer.
Huang L et al.·Signal Transduct Target Ther
2021Review
Targeting KRAS in pancreatic cancer.
Stickler S et al.·Oncol Res
2024Review
Targeting the MAPK Pathway in KRAS-Driven Tumors.
Drosten M et al.·Cancer Cell
2020Review
Top 5 results · since 2015Search PubMed ↗