KLKB1

Chr 4AR

kallikrein B1

Also known as: KLK3, PKK, PKKD, PPK

The protein encoded by this gene is a serine protease that participates in surface-dependent activation of blood coagulation, activates coagulation factor XII, and releases bradykinin from high molecular weight kininogen. Mutations cause Fletcher factor (prekallikrein) deficiency with autosomal recessive inheritance. The gene shows very low constraint to loss-of-function variants (pLI near zero), consistent with the recessive inheritance pattern of the associated bleeding disorder.

OMIMResearchSummary from RefSeq, OMIM, UniProt
MultiplemechanismARLOEUF 1.001 OMIM phenotype
Clinical SummaryKLKB1
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Gene-Disease Validity (ClinGen)
inherited prekallikrein deficiency · ARDefinitive

Definitive — sufficient evidence for diagnostic panels

Population Constraint (gnomAD)
Low constraint (pLI 0.00) — loss-of-function variants are relatively tolerated in the population.
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Clinical Trials
1 active or recruiting trial — potential therapeutic options may be available

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint
1.00LOEUF
pLI 0.000
Z-score 1.54
OE 0.71 (0.521.00)
Tolerant

Highly tolerant — LoF variants common in population

Missense Constraint
0.70Z-score
OE missense 0.89 (0.810.98)
302 obs / 338.2 exp
Tolerant

Mild missense constraint

Observed / Expected Ratios
LoF OE0.71 (0.521.00)
00.351.4
Missense OE0.89 (0.810.98)
00.61.4
Synonymous OE1.19
01.21.6
LoF obs/exp: 24 / 33.6Missense obs/exp: 302 / 338.2Syn Z: -1.62
DN
0.7036th %ile
GOF
0.6345th %ile
LOF
0.3066th %ile

This gene has evidence for multiple mechanisms of pathogenicity (dominant-negative and gain-of-function). Both the Badonyi & Marsh prediction and the broader genomic evidence point to dominant-negative as the predominant mechanism. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

DNprediction above median
GOFprediction above median

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

0 submitted variants in ClinVar

Protein Context — Lollipop Plot

KLKB1 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

3D Protein StructureAlphaFold
Clinical Literature
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