HRAS

Chr 11AD

HRas proto-oncogene, GTPase

Also known as: C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1

The HRAS protein functions in signal transduction pathways by binding GTP and GDP with intrinsic GTPase activity, undergoing continuous cycles of palmitoylation that regulate its exchange between the plasma membrane and Golgi apparatus. Germline mutations cause Costello syndrome, an autosomal dominant disorder characterized by prenatal overgrowth followed by postnatal growth deficiency, cognitive disability, distinctive facial features, cardiovascular abnormalities, and tumor predisposition. The pathogenic mechanism involves gain-of-function mutations that disrupt normal cellular signaling.

GeneReviewsOMIMResearchSummary from RefSeq, OMIM, UniProt, Mechanism
GOFmechanismADLOEUF 0.937 OMIM phenotypes
VCEP Guidelines: RASopathyReleased
View SpecificationsClinGen Panel
Clinical SummaryHRAS
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Gene-Disease Validity (ClinGen)
Costello syndrome · ADDefinitive

Definitive — sufficient evidence for diagnostic panels

2 total gene-disease associations curated

Population Constraint (gnomAD)
Low constraint (pLI 0.08) — loss-of-function variants are relatively tolerated in the population.
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ClinVar Variants
36 unique Pathogenic / Likely Pathogenic· 202 VUS of 500 total submissions
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Clinical Trials
10 active or recruiting trials — potential therapeutic options may be available
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GeneReview available — HRAS
Authoritative clinical overview · Recommended first read
Open GeneReview ↗
Some data sources returned errors (1)

opentargets: TimeoutError: The operation was aborted due to timeout

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint
0.93LOEUF
pLI 0.080
Z-score 1.72
OE 0.36 (0.160.93)
Tolerant

Typical tolerance to LoF variation

Missense Constraint
1.51Z-score
OE missense 0.62 (0.510.75)
75 obs / 121.9 exp
Tolerant

Mild missense constraint

Observed / Expected Ratios
LoF OE0.36 (0.160.93)
00.351.4
Missense OE0.62 (0.510.75)
00.61.4
Synonymous OE1.40
01.21.6
LoF obs/exp: 3 / 8.4Missense obs/exp: 75 / 121.9Syn Z: -2.27
Curated Mechanism (G2P)Gene2Phenotype (DDG2P) ↗
definitiveHRAS-related congenital myopathy with excess of muscle spindlesGOFAD
definitiveHRAS-related Costello syndromeGOFAD
DN
0.85top 5%
GOF
0.85top 5%
LOF
0.3067th %ile

This gene has evidence for multiple mechanisms of pathogenicity (gain-of-function and dominant-negative). Both the Badonyi & Marsh prediction and the broader genomic evidence point to gain-of-function as the predominant mechanism. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

GOFprediction above median · 1 literature citation
DNprediction above median · 1 literature citation

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Literature Evidence

DNAnother mutant, the Ser17Arg variant, has been described as a dominant-negative form of p21 protein.PMID:14500341
GOFHeterozygous germline mutations in the proto-oncogene HRAS cause Costello syndrome, and its inheritance pattern would thus be autosomal dominant.PMID:22488832

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

500 submitted variants in ClinVar

Classification Summary

Pathogenic22
Likely Pathogenic14
VUS202
Likely Benign214
Benign29
Conflicting14
22
Pathogenic
14
Likely Pathogenic
202
VUS
214
Likely Benign
29
Benign
14
Conflicting

Curated Variants Distribution

Classified variants from ClinVar · 5 ACMG categories

ClassificationLoFMissense + InframeNon-codingSynonymousTotal
Pathogenic
0
7
15
0
22
Likely Pathogenic
0
8
6
0
14
VUS
29
138
33
2
202
Likely Benign
3
39
74
98
214
Benign
0
1
28
0
29
Conflicting
14
Total32193156100495

LoF = frameshift, stop gained/lost, canonical splice · Counts from ClinVar esearch · Updated hourly

View in ClinVar →

Protein Context — Lollipop Plot

HRAS · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

3D Protein StructureAlphaFold

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

Non-small Cell Lung CancerHistiocytic NeoplasmHistiocytosis

Phase 1/2 Trial of S241656 in Selected RAS/MAPK Mutation- Positive Malignancies

RECRUITING
NCT05786924Phase PHASE1, PHASE2Institut de Recherches Internationales ServierStarted 2023-04-18
S241656FOLFOX6/FOLFOX7FOLFIRI
RASopathy

Study of the Thyroid Function and Echostructural Morphology in Patients Affected With Rasopathies (ECORAS2023)

RECRUITING
NCT06489067University of Bari Aldo MoroStarted 2024-01-15
Malignant Solid Neoplasms

Adapting Treatment to the Tumor Molecular Alterations for Patients With Advanced Solid Tumors: MyOwnSpecificTreatment

RECRUITING
NCT02029001Phase PHASE2Centre Leon BerardStarted 2014-03
Nilotinib (400 mg BID)Everolimus (10 mg QD)Sorafenib (400 mg BID)
Non Small Cell Lung CancerEGFR Gene MutationALK Gene Mutation

Treatment Strategies and Survival Outcome for Non-small Cell Lung Cancer With Oncogenic Mutation

RECRUITING
NCT04322890Phase PHASE2Hunan Province Tumor HospitalStarted 2020-04-16
OsimertinibAlectinib 150 MGCrizotinib 250 MG
Malignant Solid NeoplasmRecurrent Adrenal Gland PheochromocytomaRecurrent Ectomesenchymoma

Tipifarnib for the Treatment of Advanced Solid Tumors, Lymphoma, or Histiocytic Disorders With HRAS Gene Alterations, a Pediatric MATCH Treatment Trial

ACTIVE NOT RECRUITING
NCT04284774Phase PHASE2National Cancer Institute (NCI)Started 2020-10-13
Tipifarnib
Colorectal Cancer Metastatic

Exploration of Therapeutic Strategies for NeoRAS Wild-type Metastatic Colorectal Cancer Based on Circulating Tumor DNA

RECRUITING
NCT06440902Phase PHASE2Fudan UniversityStarted 2023-12-28
Cetuximab
Pancreatic CancerGastric CancerGastrointestinal Cancer

Administering Peripheral Blood Lymphocytes Transduced With a Murine T-Cell Receptor Recognizing the G12V Variant of Mutated RAS in HLA-A*11:01 Patients

RECRUITING
NCT03190941Phase PHASE1, PHASE2National Cancer Institute (NCI)Started 2017-09-21
CyclophosphamideFludarabineAnti-KRAS G12V mTCR PBL
Advanced Malignant Solid NeoplasmAnn Arbor Stage III Non-Hodgkin LymphomaAnn Arbor Stage IV Non-Hodgkin Lymphoma

Targeted Therapy Directed by Genetic Testing in Treating Pediatric Patients With Relapsed or Refractory Advanced Solid Tumors, Non-Hodgkin Lymphomas, or Histiocytic Disorders (The Pediatric MATCH Screening Trial)

ACTIVE NOT RECRUITING
NCT03155620Phase PHASE2National Cancer Institute (NCI)Started 2017-07-31
Biopsy ProcedureBiospecimen CollectionBone Marrow Aspiration and Biopsy
Colo-rectal CancerMolecular Pathway Deregulation

Clinicopathological Characteristics of Colon Cancer in Young Age

RECRUITING
NCT05365412Seoul National University Bundang HospitalStarted 2019-01-01
Gastric CancerHealthy

Preliminary Experimental Study on Key Technologies for Early Screening of Gastric Cancer

RECRUITING
NCT05991947Zhejiang Cancer HospitalStarted 2021-03-01
No intervention
Clinical Literature
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Key Publications
Landmark & review papers · by relevance
PubMed
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