EZH2

Chr 7AD

enhancer of zeste 2 polycomb repressive complex 2 subunit

Also known as: ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]

OMIMResearchGenerating clinical summary…
MultiplemechanismADLOEUF 0.151 OMIM phenotype
Clinical SummaryEZH2
🧬
Gene-Disease Validity (ClinGen)
Weaver syndrome · ADDefinitive

Definitive — sufficient evidence for diagnostic panels

Population Constraint (gnomAD)
Highly constrained gene — heterozygous loss-of-function variants are very rare in the population (pLI 1.00). One damaged copy is likely sufficient to cause disease.
💊
Clinical Trials
12 active or recruiting trials — potential therapeutic options may be available

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Dual constrained — LoF & missense intolerant
LoF Constraint?
0.15LOEUF
pLI 1.000
Z-score 5.81
OE 0.05 (0.020.15)
Highly constrained

Among the most LoF-intolerant genes (~top 3%)

Missense Constraint?
4.68Z-score
OE missense 0.36 (0.320.41)
154 obs / 425.8 exp
Constrained

Highly missense-constrained (top ~0.1%)

Observed / Expected Ratios?
LoF OE?0.05 (0.020.15)
00.351.4
Missense OE?0.36 (0.320.41)
00.61.4
Synonymous OE?1.12
01.21.6
LoF obs/exp: 2 / 43.2Missense obs/exp: 154 / 425.8Syn Z: -1.18
Curated Mechanism (G2P)Gene2Phenotype (DDG2P) ↗
definitiveEZH2-related Weaver syndromeOTHERAD

This gene — mechanism propensity

DN
0.3097th %ile
GOF
0.4776th %ile
LOF
0.82top 5%

This gene has evidence for multiple mechanisms of pathogenicity (loss-of-function and gain-of-function). Both the Badonyi & Marsh prediction and the broader genomic evidence point to loss-of-function as the predominant mechanism. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

LOFprediction above median · 1 literature citation · LOEUF 0.15
GOF1 literature citation

Literature Evidence

GOFRecurrent somatic heterozygous gain-of-function mutations of EZH2 have been identified in DLBCL, most notably affecting tyrosine 641 (Y641), inducing hyper-trimethylation of H3K27 (H3K27me3).1
LOFThe phenotype of EZH2 haploinsufficiency-1.2-Mb deletion at 7q36.1 in a child with tall stature and intellectual disability.2

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312. Mechanism ranking also informed by gnomAD constraint, ClinVar, and ClinGen data.

ClinVar Variant Classifications

0 submitted variants in ClinVar

Protein Context — Lollipop Plot

EZH2 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

Peripheral T Cells Lymphoma (PTCL)

Zeprumetostat, Azacitidine Combined With Lipo-MIT in R/R PTCL

RECRUITING
NCT07372352Phase PHASE2The First Affiliated Hospital of Soochow UniversityStarted 2026-01-15
ZeprumetostatAzacitidine (AZA)Mitoxantrone Hydrochloride Liposome
Polycythemia VeraEssential ThrombocythaemiaMyelofibrosis

Prevalence Of Germline Gene Mutations In Patients With Myeloproliferative Neoplasms With Family History

NOT YET RECRUITING
NCT06923670Phase NAFondazione Policlinico Universitario Agostino Gemelli IRCCSStarted 2025-05-21
NGS testingNGS analysis for mutations in genes involved in familial predisposition to hematological malignancies
Myeloproliferative Neoplasm

Impact of Epigenetic Age on Clinic-biological Presentation and Prognosis in Myeloproliferative Neoplasms Epigenetic Age in Myeloproliferative Neoplasms (EpiC)

RECRUITING
NCT06022328University Hospital, BordeauxStarted 2023-12-15
Assessment of the epigenetic age
Lymphoma, Follicular

A Study of Tazemetostat on Safety in Participants With Relapsed or Refractory Follicular Lymphoma With Enhancer of Zeste Homolog 2 (EZH2) Gene Mutation in Japan

ACTIVE NOT RECRUITING
NCT05228158Eisai Co., Ltd.Started 2021-08-16
Tazemetostat
Relapsed or Refractory Mature Lymphoid Neoplasms

A Phase 1 Study of SHR2554 in Subjects With Relapsed or Refractory Mature Lymphoid Neoplasms

ACTIVE NOT RECRUITING
NCT03603951Phase PHASE1Jiangsu HengRui Medicine Co., Ltd.Started 2018-08-14
SHR2554
Locally Advanced Urothelial CarcinomaMetastatic Urothelial CarcinomaStage III Bladder Cancer AJCC v8

Testing the Addition of Tazemetostat to the Immunotherapy Drug, Pembrolizumab (MK-3475), in Advanced Urothelial Carcinoma

ACTIVE NOT RECRUITING
NCT03854474Phase PHASE1, PHASE2National Cancer Institute (NCI)Started 2019-11-18
Biospecimen CollectionComputed TomographyMagnetic Resonance Imaging
Advanced Malignant Solid NeoplasmAnn Arbor Stage III Non-Hodgkin LymphomaAnn Arbor Stage IV Non-Hodgkin Lymphoma

Targeted Therapy Directed by Genetic Testing in Treating Pediatric Patients With Relapsed or Refractory Advanced Solid Tumors, Non-Hodgkin Lymphomas, or Histiocytic Disorders (The Pediatric MATCH Screening Trial)

ACTIVE NOT RECRUITING
NCT03155620Phase PHASE2National Cancer Institute (NCI)Started 2017-07-31
Biopsy ProcedureBiospecimen CollectionBone Marrow Aspiration and Biopsy
Chronic Myelomonocytic Leukemia

Relevance of Peripheral Cells in the Pathophysiology of Chronic Myelomonocytic Leukemia (CMML)

RECRUITING
NCT03280888Centre Hospitalier Universitaire de NiceStarted 2014-11-05
Solid Tumor MalignanciesClear Cell Endometrial CarcinomaOvarian Cancer

Efficacy and Safety of the Valemetostat in Patients With Selected Solid Tumors.

RECRUITING
NCT07303387Phase PHASE2Gustave Roussy, Cancer Campus, Grand ParisStarted 2026-03-05
Valemetostat Tosylate
PTCLRefractory

Genotype-guided Targeted Agents Plus EZH2i for Primary Refractory PTCL

NOT YET RECRUITING
NCT07493330Phase PHASE1, PHASE2Ruijin HospitalStarted 2026-03-23
Zeprumetostat+AzacitadineZeprumetostat+DecitabineZeprumetostat+Chidamide
Clinical Stage IV Cutaneous Melanoma AJCC v8Metastatic Melanoma

Testing the Addition of the Anti-cancer Drug, Tazemetostat, to the Usual Treatment (Dabrafenib and Trametinib) for Metastatic Melanoma That Has Progressed on the Usual Treatment

ACTIVE NOT RECRUITING
NCT04557956Phase PHASE1, PHASE2National Cancer Institute (NCI)Started 2021-08-19
Biopsy ProcedureComputed TomographyDabrafenib Mesylate
AMLMDS

Molecular Genetics Guide the Maintenance Therapy After Allogeneic Hematopoietic Stem Cell Transplantation

RECRUITING
NCT06972641Phase PHASE2, PHASE3Ruijin HospitalStarted 2025-06-10
DecitabineSorafenib (BAY-43-9006),giritinibAvastinib