EZH2

Chr 7AD

enhancer of zeste 2 polycomb repressive complex 2 subunit

Also known as: ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2

The protein serves as the catalytic subunit of the PRC2 complex, methylating histone H3 at lysine 27 to repress gene transcription, which is essential for embryonic development and stem cell identity. Mutations cause Weaver syndrome, characterized by overgrowth, intellectual disability, and distinctive facial features with autosomal dominant inheritance. The gene is highly constrained against loss-of-function variants (pLI ~1.0), reflecting its critical role in development.

OMIMResearchSummary from RefSeq, OMIM, UniProt
MultiplemechanismADLOEUF 0.151 OMIM phenotype
Clinical SummaryEZH2
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Gene-Disease Validity (ClinGen)
Weaver syndrome · ADDefinitive

Definitive — sufficient evidence for diagnostic panels

Population Constraint (gnomAD)
Highly constrained gene — heterozygous loss-of-function variants are very rare in the population (pLI 1.00). One damaged copy is likely sufficient to cause disease.
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Clinical Trials
12 active or recruiting trials — potential therapeutic options may be available

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Dual constrained — LoF & missense intolerant
LoF Constraint
0.15LOEUF
pLI 1.000
Z-score 5.81
OE 0.05 (0.020.15)
Highly constrained

Among the most LoF-intolerant genes (~top 3%)

Missense Constraint
4.68Z-score
OE missense 0.36 (0.320.41)
154 obs / 425.8 exp
Constrained

Highly missense-constrained (top ~0.1%)

Observed / Expected Ratios
LoF OE0.05 (0.020.15)
00.351.4
Missense OE0.36 (0.320.41)
00.61.4
Synonymous OE1.12
01.21.6
LoF obs/exp: 2 / 43.2Missense obs/exp: 154 / 425.8Syn Z: -1.18
Curated Mechanism (G2P)Gene2Phenotype (DDG2P) ↗
definitiveEZH2-related Weaver syndromeOTHERAD
DN
0.3097th %ile
GOF
0.4776th %ile
LOF
0.82top 5%

This gene has evidence for multiple mechanisms of pathogenicity (loss-of-function and gain-of-function). Both the Badonyi & Marsh prediction and the broader genomic evidence point to loss-of-function as the predominant mechanism. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

LOFprediction above median · 1 literature citation · LOEUF 0.15
GOF1 literature citation

Literature Evidence

GOFRecurrent somatic heterozygous gain-of-function mutations of EZH2 have been identified in DLBCL, most notably affecting tyrosine 641 (Y641), inducing hyper-trimethylation of H3K27 (H3K27me3).PMID:25762637
LOFThe phenotype of EZH2 haploinsufficiency-1.2-Mb deletion at 7q36.1 in a child with tall stature and intellectual disability.PMID:28696078

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312. Mechanism ranking also informed by gnomAD constraint, ClinVar, and ClinGen data.

ClinVar Variant Classifications

0 submitted variants in ClinVar

Protein Context — Lollipop Plot

EZH2 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

3D Protein StructureAlphaFold

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

Myeloproliferative Neoplasms

Registry of Patients With MPNs in Taiwan

ACTIVE NOT RECRUITING
NCT03618485Chang Gung Memorial HospitalStarted 2017-04-01
Solid Tumor MalignanciesClear Cell Endometrial CarcinomaOvarian Cancer

Efficacy and Safety of the Valemetostat in Patients With Selected Solid Tumors.

RECRUITING
NCT07303387Phase PHASE2Gustave Roussy, Cancer Campus, Grand ParisStarted 2026-03-05
Valemetostat Tosylate
Polycythemia VeraEssential ThrombocythaemiaMyelofibrosis

Prevalence Of Germline Gene Mutations In Patients With Myeloproliferative Neoplasms With Family History

NOT YET RECRUITING
NCT06923670Phase NAFondazione Policlinico Universitario Agostino Gemelli IRCCSStarted 2025-05-21
NGS testingNGS analysis for mutations in genes involved in familial predisposition to hematological malignancies
AMLMDS

Molecular Genetics Guide the Maintenance Therapy After Allogeneic Hematopoietic Stem Cell Transplantation

RECRUITING
NCT06972641Phase PHASE2, PHASE3Ruijin HospitalStarted 2025-06-10
DecitabineSorafenib (BAY-43-9006),giritinibAvastinib
Myeloproliferative Neoplasm

Impact of Epigenetic Age on Clinic-biological Presentation and Prognosis in Myeloproliferative Neoplasms Epigenetic Age in Myeloproliferative Neoplasms (EpiC)

RECRUITING
NCT06022328University Hospital, BordeauxStarted 2023-12-15
Assessment of the epigenetic age
Peripheral T-Cell Lymphoma

Real-world Outcomes of Peripheral T-cell Lymphoma: A Multicenter Retrospective and Prospective Cohort Study

RECRUITING
NCT07270861Fudan UniversityStarted 2025-11-15
Observational
Locally Advanced Urothelial CarcinomaMetastatic Urothelial CarcinomaStage III Bladder Cancer AJCC v8

Testing the Addition of Tazemetostat to the Immunotherapy Drug, Pembrolizumab (MK-3475), in Advanced Urothelial Carcinoma

ACTIVE NOT RECRUITING
NCT03854474Phase PHASE1, PHASE2National Cancer Institute (NCI)Started 2019-11-18
Biospecimen CollectionComputed TomographyMagnetic Resonance Imaging
Lymphoma, Follicular

A Study of Tazemetostat on Safety in Participants With Relapsed or Refractory Follicular Lymphoma With Enhancer of Zeste Homolog 2 (EZH2) Gene Mutation in Japan

ACTIVE NOT RECRUITING
NCT05228158Eisai Co., Ltd.Started 2021-08-16
Tazemetostat
Recurrent B-Cell Non-Hodgkin LymphomaRecurrent Diffuse Large B-Cell Lymphoma Germinal Center B-Cell TypeRecurrent Follicular Lymphoma

Testing the Safety of the Anti-cancer Drugs Tazemetostat and Belinostat in Patients With Lymphomas That Have Resisted Treatment

ACTIVE NOT RECRUITING
NCT05627245Phase PHASE1National Cancer Institute (NCI)Started 2023-03-01
BelinostatBiopsy ProcedureBiospecimen Collection
Advanced Solid TumorDiffuse Large B Cell LymphomaLymphoma, T-Cell

A Study of Tulmimetostat DZR123 (CPI-0209) in Patients With Advanced Solid Tumors and Lymphomas

RECRUITING
NCT04104776Phase PHASE1, PHASE2Novartis PharmaceuticalsStarted 2019-09-18
TulmimetostatEnzalutamide
Relapsed or Refractory Mature Lymphoid Neoplasms

A Phase 1 Study of SHR2554 in Subjects With Relapsed or Refractory Mature Lymphoid Neoplasms

ACTIVE NOT RECRUITING
NCT03603951Phase PHASE1Jiangsu HengRui Medicine Co., Ltd.Started 2018-08-14
SHR2554
Advanced Malignant Solid NeoplasmAnn Arbor Stage III Non-Hodgkin LymphomaAnn Arbor Stage IV Non-Hodgkin Lymphoma

Targeted Therapy Directed by Genetic Testing in Treating Pediatric Patients With Relapsed or Refractory Advanced Solid Tumors, Non-Hodgkin Lymphomas, or Histiocytic Disorders (The Pediatric MATCH Screening Trial)

ACTIVE NOT RECRUITING
NCT03155620Phase PHASE2National Cancer Institute (NCI)Started 2017-07-31
Biopsy ProcedureBiospecimen CollectionBone Marrow Aspiration and Biopsy
Clinical Literature
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Full-Text Mentions
NLP-detected gene mentions in article bodies · via PubTator3
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Top 5 full-text resultsSearch PubTator3 ↗
Key Publications
Landmark & review papers · by relevance
PubMed
Top 5 results · since 2015Search PubMed ↗