ENTPD4

Chr 8

ectonucleoside triphosphate diphosphohydrolase 4

Also known as: LALP70, LAP70, LYSAL1, NTPDase-4, UDPase

The protein functions as an endo-apyrase that catalyzes the hydrolysis of nucleoside triphosphates and diphosphates in a calcium- or magnesium-dependent manner, with preference for pyrimidines like UTP and TTP, and may be involved in nucleotide salvage from lysosomes. Mutations cause spastic paraplegia 35, an autosomal recessive hereditary spastic paraplegia presenting with progressive lower limb spasticity. The gene shows no constraint against loss-of-function variants, consistent with recessive inheritance where heterozygous carriers are typically unaffected.

Summary from RefSeq, UniProt
Research Assistant →
0
Active trials
5
Pubs (1 yr)
81
P/LP submissions
0%
P/LP missense
1.02
LOEUF
DN
Mechanism· predicted
Clinical SummaryENTPD4
Population Constraint (gnomAD)
Low constraint (pLI 0.00) — loss-of-function variants are relatively tolerated in the population.
📋
ClinVar Variants
81 unique Pathogenic / Likely Pathogenic· 109 VUS of 214 total submissions

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint
1.02LOEUF
pLI 0.000
Z-score 1.47
OE 0.75 (0.561.02)
Tolerant

Highly tolerant — LoF variants common in population

Missense Constraint
-0.72Z-score
OE missense 1.11 (1.021.20)
403 obs / 364.2 exp
Tolerant

Tolerant to missense variation

Observed / Expected Ratios
LoF OE0.75 (0.561.02)
00.351.4
Missense OE1.11 (1.021.20)
00.61.4
Synonymous OE1.07
01.21.6
LoF obs/exp: 30 / 40.0Missense obs/exp: 403 / 364.2Syn Z: -0.66
DN
0.6648th %ile
GOF
0.5072th %ile
LOF
0.3358th %ile

The highest-scoring mechanism for this gene is dominant-negative.

DNprediction above median

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

214 submitted variants in ClinVar

Classification Summary

Pathogenic77
Likely Pathogenic4
VUS109
Likely Benign6
77
Pathogenic
4
Likely Pathogenic
109
VUS
6
Likely Benign

Curated Variants Distribution

Classified variants from ClinVar · 5 ACMG categories

ClassificationLoFMissense + InframeNon-codingSynonymousTotal
Pathogenic
0
0
77
0
77
Likely Pathogenic
0
0
4
0
4
VUS
0
105
4
0
109
Likely Benign
0
5
0
1
6
Benign
0
0
0
0
0
Total0110851196

LoF = frameshift, stop gained/lost, canonical splice · Counts from ClinVar esearch · Updated hourly

View in ClinVar →

Protein Context — Lollipop Plot

ENTPD4 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

No active trials found for this gene.

Search ClinicalTrials.gov →
Clinical Literature
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Key Publications
Landmark & review papers · by relevance
PubMed
Top 1 results · since 2015Search PubMed ↗
Recent Gene-Specific Literature
Gene in title · MEDLINE · newest first
Europe PMC