CYB5RL

Chr 1

cytochrome b5 reductase like

The protein functions as a NADH-cytochrome b5 reductase involved in fatty acid desaturation and elongation, cholesterol biosynthesis, drug metabolism, and methemoglobin reduction in red blood cells. Mutations cause autosomal recessive congenital methemoglobinemia, which presents with cyanosis due to impaired ability to reduce methemoglobin to normal hemoglobin. This gene is highly constrained against loss-of-function variants, indicating that complete loss of protein function is likely incompatible with normal development.

ResearchSummary from RefSeq, UniProt
MultiplemechanismLOEUF 1.07
Clinical SummaryCYB5RL
Population Constraint (gnomAD)
Low constraint (pLI 0.00) — loss-of-function variants are relatively tolerated in the population.

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint
1.07LOEUF
pLI 0.000
Z-score 1.34
OE 0.65 (0.411.07)
Tolerant

Highly tolerant — LoF variants common in population

Missense Constraint
-0.23Z-score
OE missense 1.05 (0.931.18)
189 obs / 180.1 exp
Tolerant

Tolerant to missense variation

Observed / Expected Ratios
LoF OE0.65 (0.411.07)
00.351.4
Missense OE1.05 (0.931.18)
00.61.4
Synonymous OE0.90
01.21.6
LoF obs/exp: 11 / 16.9Missense obs/exp: 189 / 180.1Syn Z: 0.64
DN
0.6260th %ile
GOF
0.6541th %ile
LOF
0.2485th %ile

This gene has evidence for multiple mechanisms of pathogenicity (gain-of-function and dominant-negative). Both the Badonyi & Marsh prediction and the broader genomic evidence point to gain-of-function as the predominant mechanism. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

GOFprediction above median
DNprediction above median

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

0 submitted variants in ClinVar

Protein Context — Lollipop Plot

CYB5RL · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

3D Protein StructureAlphaFold

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

No active trials found for this gene.

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