CENPO

Chr 2

centromere protein O

Also known as: CENP-O, ICEN-36, MCM21R

The protein is a component of the interphase centromere complex that localizes to centromeres throughout the cell cycle and is required for bipolar spindle assembly, chromosome segregation, and checkpoint signaling during mitosis. Mutations cause primary microcephaly with seizures and developmental delay, following autosomal recessive inheritance. The gene shows minimal constraint against loss-of-function variants (very low pLI), consistent with the recessive inheritance pattern observed clinically.

Summary from RefSeq, UniProt
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0
Active trials
3
Pubs (1 yr)
22
P/LP submissions
0%
P/LP missense
1.30
LOEUF
DN
Mechanism· predicted
Clinical SummaryCENPO
Population Constraint (gnomAD)
Low constraint (pLI 0.00) — loss-of-function variants are relatively tolerated in the population.
📋
ClinVar Variants
21 unique Pathogenic / Likely Pathogenic· 75 VUS of 157 total submissions

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint
1.30LOEUF
pLI 0.000
Z-score 0.62
OE 0.84 (0.561.30)
Tolerant

Highly tolerant — LoF variants common in population

Missense Constraint
0.40Z-score
OE missense 0.91 (0.801.04)
148 obs / 162.5 exp
Tolerant

Mild missense constraint

Observed / Expected Ratios
LoF OE0.84 (0.561.30)
00.351.4
Missense OE0.91 (0.801.04)
00.61.4
Synonymous OE1.11
01.21.6
LoF obs/exp: 15 / 17.8Missense obs/exp: 148 / 162.5Syn Z: -0.71
DN
0.6550th %ile
GOF
0.3887th %ile
LOF
0.3162th %ile

The highest-scoring mechanism for this gene is dominant-negative.

DNprediction above median

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

157 submitted variants in ClinVar

Classification Summary

Pathogenic20
Likely Pathogenic1
VUS75
Likely Benign35
Benign5
20
Pathogenic
1
Likely Pathogenic
75
VUS
35
Likely Benign
5
Benign

Curated Variants Distribution

Classified variants from ClinVar · 5 ACMG categories

ClassificationLoFMissense + InframeNon-codingSynonymousTotal
Pathogenic
1
0
19
0
20
Likely Pathogenic
0
0
1
0
1
VUS
2
59
14
0
75
Likely Benign
1
7
7
20
35
Benign
0
0
3
2
5
Total4664422136

LoF = frameshift, stop gained/lost, canonical splice · Counts from ClinVar esearch · Updated hourly

View in ClinVar →

Protein Context — Lollipop Plot

CENPO · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

No active trials found for this gene.

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Clinical Literature
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