CDK4

Chr 12

cyclin dependent kinase 4

Also known as: CMM3, MCPH31, PSK-J3

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]

GeneReviewsResearchGenerating clinical summary…

Primary Disease Associations & Inheritance

UniProtMelanoma, cutaneous malignant 3
UniProtMicrocephaly 31, primary, autosomal recessive

Clinical highlights

Gene-disease validity (ClinGen)
melanoma, cutaneous malignant, susceptibility to, 3 · ADDefinitivesufficient evidence for diagnostic panels
Interpreting a novel variant
Curated mechanisms (Gene2Phenotype) include both loss of function and gain of function. Which applies is variant-dependent — do not assume a null variant is, or isn’t, the pathogenic class without checking the specific variant.Curated gene-level mechanism — a prior for triage, not a per-variant call.
12
Active trials
2088
Pubs (1 yr)
P/LP submissions
P/LP missense
0.64
LOEUF
Multiple*
Mechanism· G2P
📖
GeneReview available — CDK4
Authoritative clinical overview · Recommended first read
Open GeneReview ↗
Some data sources returned errors (1)

omim: Error: OMIM fetch failed: 429

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint?
0.64LOEUF
pLI 0.063
Z-score 2.61
OE 0.31 (0.160.64)
Tolerant

Typical tolerance to LoF variation

Missense Constraint?
1.49Z-score
OE missense 0.68 (0.580.79)
115 obs / 169.7 exp
Tolerant

Mild missense constraint

Observed / Expected Ratios?
LoF OE?0.31 (0.160.64)
00.351.4
Missense OE?0.68 (0.580.79)
00.61.4
Synonymous OE?1.26
01.21.6
LoF obs/exp: 5 / 16.4Missense obs/exp: 115 / 169.7Syn Z: -1.63

ClinVar Variant Classifications

0 submitted variants in ClinVar

Protein Context — Lollipop Plot

CDK4 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

Breast CancerEarly-stage Breast Cancer

Multigene Risk Score Combined With Ki-67 Dynamic Assessment in Stratified Neoadjuvant Endocrine Therapy Treatment With or Without CDK4/6 Inhibitors in HR+/HER2- Breast Cancer

ENROLLING BY INVITATION
NCT06650748Phase PHASE2Peking UniversityStarted 2025-05-01
DalpiciclibLetrozole
MelanomaSkin Cancer

A Model for Genetic Susceptibility: Melanoma

ACTIVE NOT RECRUITING
NCT00591500Memorial Sloan Kettering Cancer CenterStarted 1999-11
QuestionnaireQuestionnaire
CDK4/6 InhibitorBreast CancerCYP3A4 Protein, Human

Pharmacogenomic and Circulating Biomarkers for CDK4/6 Inhibitors

ENROLLING BY INVITATION
NCT07100054London Health Sciences Centre Research Institute OR Lawson Research Institute of St. Joseph'sStarted 2025-10-10
Whole gene sequencingDrug-drug interaction analysisBiomarker analysis
Breast Cancer

Genomic and Transcriptomic Predictors of Sequential SG Sensitivity After T-DXd in ER+/HER2-Low Metastatic Breast Cancer

RECRUITING
NCT06665178Phase PHASE2British Columbia Cancer AgencyStarted 2025-03-31
Sacituzumab Govitecan
PIK3CA MutationSolid Tumor, AdultHER2-negative Breast Cancer

First-in-Human Study of Mutant-selective PI3Kα Inhibitor, RLY-2608, as a Single Agent in Patients With Advanced Solid Tumors and in Combination With Endocrine Therapy +/- a CDK4/6 or CDK4 Inhibitor in Patients With Advanced Solid Tumors or Advanced Breast Cancer

RECRUITING
NCT05216432Phase PHASE1Relay Therapeutics, Inc.Started 2021-12-08
RLY-2608FulvestrantPalbociclib 125mg
Breast Cancer

Elacestrant and Exemestane for Patients With Pretreated HR+/HER2- Metastatic Breast Cancer and [18F] FES-avid Lesions (COMBINE)

RECRUITING
NCT07395336Phase PHASE2European Institute of OncologyStarted 2026-05-11
elacestrant and exemestane
Breast Cancer

An Open-Label, Bayesian Adaptive Phase II Clinical Study in HR+/HER2- Advanced Breast Cancer After Progression on Standard Therapy

RECRUITING
NCT07117630Phase PHASE2Fudan UniversityStarted 2025-09-25
L-Ornithine L-AspartateCDK4/6 inhibitorFulvestrant
Metastatic Solid TumorAdvanced Cancer

High Definition Medicine for Solid Tumors Oncology

RECRUITING
NCT06590506Centro Nacional de Investigaciones Oncologicas CARLOS IIIStarted 2023-03-02
Wearable
Advanced LymphomaAdvanced Malignant Solid NeoplasmBladder Carcinoma

Targeted Therapy Directed by Genetic Testing in Treating Patients With Advanced Refractory Solid Tumors, Lymphomas, or Multiple Myeloma (The MATCH Screening Trial)

ACTIVE NOT RECRUITING
NCT02465060Phase PHASE2National Cancer Institute (NCI)Started 2015-08-17
AdavosertibAfatinibAfatinib Dimaleate
Advanced Breast CancerER-positive Breast CancerHER2-negative Breast Cancer

Elacestrant + Everolimus in Patients ER+/HER2-, ESR1mut, Advanced Breast Cancer Progressing to ET and CDK4/6i.

RECRUITING
NCT06382948Phase PHASE3MedSIRStarted 2024-12-05
EverolimusElacestrantPlacebo
Locally Advanced (Inoperable) or Metastatic Breast Cancer

Capivasertib + CDK4/6i + Fulvestrant for Advanced/Metastatic HR+/HER2- Breast Cancer (CAPItello-292)

ACTIVE NOT RECRUITING
NCT04862663Phase PHASE3AstraZenecaStarted 2021-05-10
CapivasertibFulvestrantPalbociclib
Cancer

Analysis of Circulating Tumor mArkers in Blood 4 - ALCINA 4

RECRUITING
NCT05088395Phase NAInstitut CurieStarted 2022-05-19
Blood sampleBiopsy