CD274

Chr 9AR

CD274 molecule

Also known as: ADMIO5, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

OMIMResearchGenerating clinical summary…
MultiplemechanismARLOEUF 0.801 OMIM phenotype
Clinical SummaryCD274
Population Constraint (gnomAD)
Low constraint (pLI 0.02) — loss-of-function variants are relatively tolerated in the population.
📋
ClinVar Variants
1 unique Pathogenic / Likely Pathogenic· 10 VUS of 22 total submissions
💊
Clinical Trials
12 active or recruiting trials — potential therapeutic options may be available

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint?
0.80LOEUF
pLI 0.019
Z-score 2.08
OE 0.38 (0.200.80)
Tolerant

Typical tolerance to LoF variation

Missense Constraint?
0.90Z-score
OE missense 0.80 (0.690.93)
123 obs / 154.4 exp
Tolerant

Mild missense constraint

Observed / Expected Ratios?
LoF OE?0.38 (0.200.80)
00.351.4
Missense OE?0.80 (0.690.93)
00.61.4
Synonymous OE?1.23
01.21.6
LoF obs/exp: 5 / 13.1Missense obs/exp: 123 / 154.4Syn Z: -1.39

This gene — mechanism propensity

DN
0.80top 25%
GOF
0.75top 25%
LOF
0.1598th %ile

This gene has evidence for multiple mechanisms of pathogenicity (dominant-negative and gain-of-function). Both the Badonyi & Marsh prediction and the broader genomic evidence point to dominant-negative as the predominant mechanism. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

DNprediction above median
GOFprediction above median

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312.

ClinVar Variant Classifications

22 submitted variants in ClinVar

Classification Summary

Pathogenic1
VUS10
Likely Benign2
Benign5
1
Pathogenic
10
VUS
2
Likely Benign
5
Benign

Curated Variants Distribution

Classified variants from ClinVar · 5 ACMG categories

ClassificationLoFMissense + InframeNon-codingSynonymousTotal
Pathogenic
0
0
1
0
1
Likely Pathogenic
0
0
0
0
0
VUS
0
10
0
0
10
Likely Benign
0
2
0
0
2
Benign
0
0
3
2
5
Total0124218

LoF = frameshift, stop gained/lost, canonical splice · Counts from ClinVar esearch · Updated hourly

View in ClinVar →

163 pathogenic / likely-pathogenic (of 173) ClinVar copy-number / structural variants overlap CD274 — these span large chromosomal regions, not the gene specifically, and are excluded from the counts above. Explore in CNV tools →

Protein Context — Lollipop Plot

CD274 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

Carcinoma, Non-Small-Cell LungNeoplasm Metastasis

A Study of First-Line Olomorasib (LY3537982) and Pembrolizumab With or Without Chemotherapy in Patients With Advanced KRAS G12C-Mutant Non-small Cell Lung Cancer

RECRUITING
NCT06119581Phase PHASE3Eli Lilly and CompanyStarted 2023-12-21
LY3537982PembrolizumabPlacebo
Colorectal Cancer (CRC)Tumor Infiltrating LymphocytesPD-1

Study on the Safety and Tolerability of PD-1 Knockout Tumor-infiltrating T Cells (TILs) in the Treatment of Advanced Colorectal Cancer

RECRUITING
NCT07035002Phase PHASE1Ruijin HospitalStarted 2024-12-31
transfusion of 5×10^8 PD-1 knockout TILs per kg body weighttransfusion of 1×10^9 PD-1 edited TILs per kg body weighttransfusion of 2×10^9 PD-1 edited TILs per kg body weight
Carcinoma, Non-Small-Cell Lung

MYLUNG Consortium Study Protocol 2

ACTIVE NOT RECRUITING
NCT05644808US Oncology ResearchStarted 2020-12-29
Lung Cancer (NSCLC)

An Open-label, Single-arm Clinical Study of Stapokibart Injection in Combination with Tislelizumab Injection in Patients with Non-Small Cell Lung Cancer

NOT YET RECRUITING
NCT06883552Phase PHASE2Sichuan UniversityStarted 2025-10-01
Tislelizumab Injection
Recurrent Head and Neck Squamous Cell CarcinomaMetastatic Head and Neck Squamous Cell Carcinoma

Olaparib in Combination With Pembrolizumab and Carboplatin as First-Line Treatment of Recurrent or Metastatic Head and Neck Squamous-Cell Carcinoma

ACTIVE NOT RECRUITING
NCT04643379Phase PHASE2Washington University School of MedicineStarted 2021-08-07
OlaparibPembrolizumabCarboplatin
NSCLC

A Phase Ib Study of Tislelizumab Plus SYS6010 in Immunotherapy-Pretreated Locally Advanced or Metastatic NSCLC

NOT YET RECRUITING
NCT07692048Phase PHASE1Sichuan UniversityStarted 2026-06-30
SYS6010
Lung Adenocarcinoma

PHOENIX: QL1706 Plus Chemotherapy and Bevacizumab in AGA-Resistant, PD-L1 ≥50% Non-Squamous NSCLC

NOT YET RECRUITING
NCT07416058Phase PHASE2Guangdong Association of Clinical TrialsStarted 2026-01-31
QL1706 (bispecific antibody targeting PD-1 and CLTA-4)
NSCLC Stage IIIA/BNSCLC Stage II

Construction of a Prognostic and Prediction Model for Perioperative Immunotherapy in NSCLC: A Multi - Omics Perspective

NOT YET RECRUITING
NCT06856798Ziming LiStarted 2025-03-10
Solid Tumor, Adult

Tiragolumab and Atezolizumab in Advanced Pan-cancer Patients

RECRUITING
NCT06003621Phase PHASE2OmicoStarted 2023-12-15
TiragolumabAtezolizumab
Head and Neck CancerUrologic CancerLung Cancer

In-depth Characterization of Circulating and Infiltrating Immune Subsets and Tumor Cells in Cancer Patients

NOT YET RECRUITING
NCT06827639Assistance Publique Hopitaux De MarseilleStarted 2025-04-30
blood collectiontissue specimen collection
Advanced Solid TumorsMetastatic Solid Tumors

NP137 Clinical and Biological Activities Assessment in Patients With Advanced/Metastatic Solid Tumors Treated by Standard Anti PD-1/PD-L1 Immunotherapies

ACTIVE NOT RECRUITING
NCT05605496Phase PHASE2Centre Leon BerardStarted 2023-01-06
NP137anti-PD-1/PD-L1
Non-small Cell Lung Cancer

A Trial to Learn How the Combination of Fianlimab With Cemiplimab and Chemotherapy Works Compared With Cemiplimab and Chemotherapy for Treating Adult Patients With Advanced Non-small Cell Lung Cancer

ACTIVE NOT RECRUITING
NCT05800015Phase PHASE2, PHASE3Regeneron PharmaceuticalsStarted 2023-08-08
fianlimabcemiplimabPemetrexed