BRCA2

Chr 13ADSomaticAR

BRCA2 DNA repair associated

Also known as: BRCC2, BROVCA2, FACD, FAD, FAD1, FANCD, FANCD1, GLM3

Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The largest exon in both genes is exon 11, which harbors the most important and frequent mutations in breast cancer patients. The BRCA2 gene was found on chromosome 13q12.3 in human. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, May 2020]

Primary Disease Associations & Inheritance

{Breast cancer, male, susceptibility to}MIM #114480
ADSomatic
{Breast-ovarian cancer, familial, 2}MIM #612555
AD
{Glioblastoma 3}MIM #613029
AR
{Medulloblastoma}MIM #155255
ADARSomatic
{Pancreatic cancer 2}MIM #613347
{Prostate cancer}MIM #176807
ADSomatic
Fanconi anemia, complementation group D1MIM #605724
AR
Wilms tumorMIM #194070
ADSomatic
UniProtFanconi anemia complementation group D1
UniProtGlioma 3
12
Active trials
155
Pathogenic / LP
700
ClinVar variants
5
Pubs (1 yr)
-1.3
Missense Z
0.64
LOEUF
Clinical SummaryBRCA2
🧬
Gene-Disease Validity (ClinGen)
Fanconi anemia complementation group D1 · ARDefinitive

Definitive — sufficient evidence for diagnostic panels

2 total gene-disease associations curated

Population Constraint (gnomAD)
Low constraint (pLI 0.00) — loss-of-function variants are relatively tolerated in the population.
📋
ClinVar Variants
155 Pathogenic / Likely Pathogenic· 344 VUS of 700 total submissions
💊
Clinical Trials
12 active or recruiting trials — potential therapeutic options may be available
📖
GeneReview available — BRCA2
Authoritative clinical overview · Recommended first read
Open GeneReview ↗

Population Genetics & Constraint

gnomAD v4 — loss-of-function & missense intolerance

Tolerant — LoF & missense variants common in population
LoF Constraint?LOEUF (Loss-of-function Observed/Expected Upper bound Fraction) is the upper bound of the 90% CI for LoF OE — the preferred gnomAD v4 metric. Lower = more intolerant to LoF. LOEUF < 0.35 = highly constrained.
0.64LOEUF
pLI 0.000
Z-score 4.92
OE 0.51 (0.420.64)
Tolerant

Typical tolerance to LoF variation

Missense Constraint?Missense Z-score: standard deviations fewer missense variants observed vs. expected. Z > 3.09 (p < 0.001) = gene does not tolerate missense variation. OE missense < 0.6 is also considered constrained.
-1.29Z-score
OE missense 1.09 (1.051.13)
1812 obs / 1664.0 exp
Tolerant

Tolerant to missense variation

Observed / Expected Ratios?Shaded band = 90% confidence interval. Vertical tick = point estimate. Grey threshold line = gnomAD constraint cutoff for that variant class.
LoF OE?Ratio of observed to expected LoF variants. Upper CI bound (LOEUF) ≤ 0.35 = strong LoF constraint signal.0.51 (0.420.64)
00.351.4
Missense OE?Ratio of observed to expected missense variants. OE ≤ 0.6 = fewer missense variants than expected by chance.1.09 (1.051.13)
00.61.4
Synonymous OE?Control metric — synonymous variants are largely neutral and expected near OE = 1.0. Significant deviation may indicate annotation issues.0.99
01.21.6
LoF obs/exp: 61 / 118.9Missense obs/exp: 1812 / 1664.0Syn Z: 0.21
DN
0.6259th %ile
GOF
0.2597th %ile
LOF
0.4528th %ile

This gene has evidence for multiple mechanisms of pathogenicity (dominant-negative and loss-of-function). Both the Badonyi & Marsh prediction and the broader genomic evidence point to dominant-negative as the predominant mechanism. Different variants in this gene may act through different mechanisms — interpret in context of the specific variant.

DNprediction above median · 1 literature citation
LOF1 literature citation · 75% of P/LP variants are LoF

Note: In-silico variant effect predictors (SIFT, PolyPhen, REVEL, CADD) may underestimate pathogenicity of missense variants in genes with GOF or DN mechanisms. Consider functional evidence and clinical context.

Literature Evidence

DNWe further discuss two non-mutually exclusive potential scenarios related to the resulting haploinsufficiency and variant-specific dominant negative phenotype that might explain, at least in part, the variable expressivity associated with BRCA2 pathogenic variants.PMID:30803554
LOFLarge genomic deletions inactivate the BRCA2 gene in breast cancer familiesPMID:16199546

Predictions from Badonyi M, Marsh JA. PLoS ONE. 2024;19(8):e0307312. Mechanism ranking also informed by gnomAD constraint, ClinVar, and ClinGen data.

ClinVar Variant Classifications

700 submitted variants in ClinVar

Classification Summary

Pathogenic141
Likely Pathogenic14
VUS344
Likely Benign193
Benign5
Conflicting3
141
Pathogenic
14
Likely Pathogenic
344
VUS
193
Likely Benign
5
Benign
3
Conflicting

Curated Variants Distribution

Classified variants from ClinVar · 5 ACMG categories

ClassificationLoFMissense + InframeNon-codingSynonymousTotal
Pathogenic
105
1
35
0
141
Likely Pathogenic
11
0
3
0
14
VUS
5
306
29
4
344
Likely Benign
1
20
53
119
193
Benign
0
2
3
0
5
Conflicting
3
Total122329123123700

LoF = frameshift, stop gained/lost, canonical splice · Counts from ClinVar esearch · Updated hourly

View in ClinVar →

Protein Context — Lollipop Plot

BRCA2 · protein map & ClinVar variants

Showing all ClinVar variants across the protein. Search a specific variant to highlight its position.

Gene2Phenotype Curations

BRCA2-related Fanconi anemia

definitive
ARLoss Of FunctionAbsent Gene Product
Dev. DisordersSkin
G2P ↗

BRCA2-related cancer

definitive
ADLoss Of FunctionAbsent Gene Product
SkinCancer
G2P ↗

Gene2Phenotype curations · DECIPHER consortium patient cohort (public variants) · deciphergenomics.org

Clinical Trials

Active and recruiting trials from ClinicalTrials.gov

Advanced Breast Cancer

Saruparib (AZD5305) Plus Camizestrant Compared With CDK4/6 Inhibitor Plus Endocrine Therapy or Plus Camizestrant in HR-Positive, HER2-Negative (IHC 0, 1+, 2+/ ISH Non-amplified), BRCA1, BRCA2, or PALB2m Advanced Breast Cancer

RECRUITING
NCT06380751Phase PHASE3AstraZenecaStarted 2024-08-01
Saruparib (AZD5305)CamizestrantAbemaciclib
Uveal Melanoma

Identification of New Candidate Genes for Hereditary Predisposition to Uveal Melanoma

ACTIVE NOT RECRUITING
NCT06550674Phase NACentre Jean PerrinStarted 2024-10-29
Constitutional exome analysis
Advanced Malignant Solid NeoplasmMetastatic Malignant Solid NeoplasmUnresectable Malignant Solid Neoplasm

Testing the Combination of the Anti-cancer Drugs ZEN003694 (ZEN-3694) and Talazoparib in Patients With Advanced Solid Tumors, The ComBET Trial

RECRUITING
NCT05327010Phase PHASE2National Cancer Institute (NCI)Started 2022-11-14
BET Bromodomain Inhibitor ZEN-3694Biopsy ProcedureBiospecimen Collection
Ovarian CarcinomaFallopian Tube CarcinomaPrimary Peritoneal Carcinoma

NGS-based Germline and Somatic Genetic Test in Ovarian Carcinoma

ACTIVE NOT RECRUITING
NCT06972693Phase PHASE4European Institute of OncologyStarted 2018-06-12
BRCA testing
Prostate CancerProstate BiopsyGenetic Counselling

The PROFILE Study: Germline Genetic Profiling: Correlation With Targeted Prostate Cancer Screening and Treatment

RECRUITING
NCT02543905Institute of Cancer Research, United KingdomStarted 2015-03-09
Prostate MRI and Biopsy
Metastatic Pancreatic Cancer

Olaparib and Durvalumab (MEDI4736) in Patients with Metastatic Pancreatic Cancer and DNA Damage Repair Genes Alterations

ACTIVE NOT RECRUITING
NCT05659914Phase PHASE2Spanish Cooperative Group for the Treatment of Digestive Tumours (TTD)Started 2022-11-28
olaparib+durvalumab
Prostate CancerBRCA1 MutationBRCA2 Mutation

Study of Neoadjuvant PARP Inhibition Followed by Radical Prostatectomy in Patients With Unfavorable Intermediate-Risk or High-Risk Prostate Cancer With Select HRR Gene Alterations

RECRUITING
NCT05498272Phase PHASE2Rana McKay, MDStarted 2023-02-01
OlaparibLHRH agonist
Breast CancerBRCA 1 Gene MutationBRCA 2 Gene Mutation

A Prospective CohorT Study of HandX - Assisted ENdoscopic MAstectomy: Feasibility and Safety (ATHENA I Study)

RECRUITING
NCT07292246Phase NAVasileios KallesStarted 2025-09-09
Endoscopic nipple sparing mastectomyHandX endoscopic instrument
Breast CancerBRCA1 MutationBRCA2 Mutation

Endocrine Disruptors and Life STILe in Breast Cancer Development

ACTIVE NOT RECRUITING
NCT05748353Fondazione Policlinico Universitario Agostino Gemelli IRCCSStarted 2012-01-01
Hereditary Breast and Ovarian CancerMutation

Investigation of Tumour Spectrum of Germline Mutations in Breast and Ovarian Cancer Genes.

ACTIVE NOT RECRUITING
NCT03246841Phase NAUNICANCERStarted 2017-09-21
Genetic testing
Pancreatic Cancer

Pancreatic Cancer Genetics

RECRUITING
NCT01102569Columbia UniversityStarted 2008-01
Prostate Cancer

Analysing Outcomes After Prostate Cancer Diagnosis and Treatment in Carriers of Rare Germline Mutations

RECRUITING
NCT02705846Institute of Cancer Research, United KingdomStarted 2014-09
Observation of treatment outcomes via Questionnaire
Clinical Literature
Landmark / reviewRecent case evidence